视网膜脱离后视网膜下液谷氨酸和γ-氨基丁酸变化及毒性分析  

Change in the content of glutamate and γ-aminobutyric acid in the subretinal fluid in patients with retinal detachment

作　　者：郑卫东[1] 谢茂松[1] 徐国兴[1] 郑玉聪[2] 邓思珊[2] 

机构地区：[1]福建医科大学附属第一医院眼科,福州350005 [2]福建省医学科学研究所,福建省医学测试重点实验室,350001

出　　处：《中国实用眼科杂志》2009年第3期235-238,共4页Chinese Journal of Practical Ophthalmology

基　　金：基金项目：福建省自然科学基金项目（C0510015）,福建医科大学教授学术发展基金（JS06033）,人事部留学回国人员优秀课题基金（GRB-06-164-1）

摘　　要：目的探讨视网膜脱离后PVR与Glu和GABA含量的关系。方法实验分对照组、非PVR组和PVR组，采用高压液相色谱仪定量检测患者血液和视网膜下液（对照组为玻璃体）中谷氨酸和γ-氨基丁酸的含量。结果视网膜脱离患者血液中的Glu和GABA与对照组相比差异无统计学意义；视网膜下液中Glu含量明显增高，且PVR组较非PVR组高，组间差异有统计学意义（F=14．031，P〈0．001）；视网膜下液中GABA含量增高，且PVR组较非PVR组高，组间差异有统计学意义（F=9．311，P=0．001）；视网膜下液中Glu／GABA明显增高，且PVR组较非PVR组高，组间差异有统计学意义（F=4．411，P=0．022）。结论视网膜脱离后GABA含量不足以拮抗Glu的毒性作用，导致视网膜三级神经元传递功能发生障碍，影响视功能。Objective To explore the relation between proliferative vitreous retinopathy after retinal detachment and Glu and GABA.Methods Patients were divided into the control group, non-PVR Group and the PVR group.The content of glutamate and γ-aminobutyric acid in the subretinal fluid （the vitreous for the control group ）and blood were quantitatively detected using high pressure liquid chromatography.Results The differences of glutamate and y -aminobutyric acid in the blood between the patients with retinal detachment and the control patients was not statistically significant.The content of glutamate in the subretinal fluid of pa- tients with retinal detachment was higher than that in the vitreous of control patients, and the content of gluta- mate in the subretinal fluid of patients with proliferative vitreous retinopathy was higher than that of patients with non- proliferative vitreous retinopathy.The differences were statistically significant（F = 14.031, P 〈0.001 ）. The content of y -aminobutyric acid in the subretinal fluid of patients with retinal detachment was higher than that in the vitreous of control patients, and the content ofy -aminobutyric acid in the subretinal fluid of patients with proliferative vitreous retinopathy was higher than that of patients with non-proliferative vitreous retinopa- thy.The differences were statistically significant （F = 9.311, P = 0.001 ）.The rate of glutamate and γ-aminobu- tyric acid in the subretinal fluid of patients with retinal detachment was higher than that in the vitreous of control patients, and the rate of glutamate and γ-aminobutyric acid in the subretinal fluid of patients with prolifer- ative vitreous retinopathy was higher than that of patients with non-proliferative vitreous retinopathy.The differ- ences were statistically significant（F = 4.411, P = 0.022 ）.Conclussion The content of γ-aminobutyric acid in the subretinal fluid is not sufficient to antagonize the glutamate toxic effect in patients with retinal detach- ment, especial in patient

关 键 词：视网膜脱离 增殖性玻璃体视网膜病变 谷氨酸(Glu) γ-氨基丁酸(GABA) 

分 类 号：R774.12[医药卫生—眼科] R338[医药卫生—临床医学]