紫芝液体发酵培养基的初步筛选和优化  

Preliminary select and optimization of submerged fermentation media of Ganoderma sinense 2003-1

作　　者：王晓玲[1] 刘高强[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410004

出　　处：《食品科技》2009年第3期14-16,共3页Food Science and Technology

基　　金：国家自然科学基金项目(30700552);湖南省教育厅科研项目(05C327)

摘　　要：目的:筛选适宜紫芝(G.sinense 2003-1)液体发酵的培养基。方法:采用不同碳源、氮源,以及磷酸二氢钾浓度进行液体发酵实验,并采用正交设计法优化各培养基组成。结果:最适宜此紫芝菌株生长的碳源为蔗糖,氮源为蛋白胨和酵母膏的组合物;正交设计优化结果表明,此紫芝菌株液体发酵的最佳培养基组成为蔗糖35g/L、蛋白胨3g/L、酵母膏1g/L、磷酸二氢钾0.7g/L、硫酸镁0.2g/L、VB10.01g/L。结论:在优化后的培养基条件下,紫芝菌体干质量可达9.3g/L。Object： To select the optimal media composition for the submerged fermentation of Ganoderma sinense 2003-1. Methods： Vadous carbon sources, nitrogen sources and the concentration of KH2PO4 on the biomass of G. sinense were tested, and orthogonal design were applied. Results： The optimal carbon source was sucrose, and nitrogen source was a combination of peptone and yeast extract; and orthogonal design results showed that the optimal media compositions were as follows： sucrose 35 g/L, peptone 3 g/L, yeast extract 1 g/L, KH2PO4 0.7 g/L, MgSO4 0.2 g/L, VB1 0.01 g/L. Conclusion： With the optimized media, the highest dry cell weight of G. sinense 2003-1 reached 9.3 g/L.

关 键 词：紫芝 液体发酵 正交设计法 

分 类 号：TS201.3[轻工技术与工程—食品科学] Q939.93[轻工技术与工程—食品科学与工程]