人工养殖鲟鱼鱼肝中辅酶Q_(10)提取工艺的研究  

Study on extraction technology of CoQ_(10) from the liver of farmed sturgeon

作　　者：户业丽[1] 韩祖晶[1] 郑伟[1] 吕中[2] 程波[3] 陈飞[1] 闫雪[1] 蓝泽桥[4] 

机构地区：[1]武汉工程大学化工与制药学院,武汉430074 [2]湖北省新型反应器与绿色化学工艺重点实验室,武汉430074 [3]绿色化工工程省部共建教育部重点实验室,武汉430074 [4]湖北天峡鲟业有限公司,宜都443300

出　　处：《食品科技》2009年第3期211-214,共4页Food Science and Technology

基　　金：2008年湖北省基础化学示范中心项目

摘　　要：对人工养殖鲟鱼鱼肝中辅酶Q10进行提取工艺的研究。人工养殖鲟鱼鱼肝经除血水、去脂肪等预处理,然后采用醇-碱皂化法提取辅酶Q10。通过正交实验,确定皂化反应的最佳工艺条件,结果表明提取辅酶Q10的最佳工艺条件是NaOH浓度为7.00%、乙醇/水体积比为100.00%、没食子酸/鱼肝质量比为7.00%、皂化时间为30min,在此条件下辅酶Q10的提取率为0.15%。对粗提液采用薄层层析法和紫外吸收法进行定性和定量分析。Extraction technology of CoQ10 in the liver of farmed sturgeon was studied. First, water and fat was removed from the liver of farmed sturgeon, and then CoQ10 was extracted by alcohol-base saponification. The orthogonal experiment design was applied to optimum the saponification. The result of this study indication that： the optimum technology was 7.00% of concentration of NaOH, 100.00% of ethanol, 7.00% of pyrogallolic acid, saponification time 30 min, the extraction content of CoQ10 was 0.15% under the said condition. Last, extract was determined by TLC and UV.

关 键 词：鲟鱼肝 辅酶Q10 提取 正交实验 

分 类 号：TS201.25[轻工技术与工程—食品科学]