RP-HPLC测定牛膝中齐墩果酸的含量  

Determination of Oleanolic acid in Achyranthes bientata B1. with RP-HPLC

作　　者：余淑娴[1] 熊世权[1] 郑湘娟[1] 罗红梅[1] 洪三国[1] 

机构地区：[1]南昌大学化学系,南昌330031

出　　处：《食品科技》2009年第3期256-258,共3页Food Science and Technology

基　　金：江西省教育厅科技计划项目(赣教技字[2006]48)

摘　　要：目的:建立牛膝中齐墩果酸含量的RP-HPLC测定方法。方法:采用超声提取方法;色谱条件:Allsphere ODS-2柱(250mm×4.6mm,5μ),流动相为甲醇-水-醋酸(80∶20∶0.01,v/v/v),柱温30℃,流速1.0mL/min,检测波长215nm。结果:齐墩果酸在0.01～0.4mg/mL范围内线性关系良好,线性方程为Y=2796327X-31842,r=0.9997,平均回收率为98.96%,RSD=0.21%(n=5),比较了不同产地牛膝中齐墩果酸的含量,其中山东产的含量最高。结论:实验方法简单、准确可靠,为牛膝的质量评价提供了实验依据。Objective： To establish an RP-HPLC method to determine the content of oleanolic acid in Achyranthes bientata BI.. Methods： By ultrasonic extraction technique, the suitable chromatogram conditions： AIIsphere ODS-2 column（250 mm×4.6 mm, 5 μ）, the mobile phase was methanol-water-acetic acid（80：20：0.01, v/v/v）, column temperature was at 30 ℃, flow rate was 1.0 mL/min, UV detection wavelength was 215 nm. Results： The good linear range of Oleanolic Acid was 0.01-0.4 mg/mL, regression equation： Y=2796327X- 27842, r=0.9997, the average recovery rate was 98.96%, RSD=0.21%（n=5）. The content of Oleanolic acid is the highest in Shandong Province, in comparison of others. Conclusion： The method is simple, accurate and can be used to evaluate the quality of Achyranthes bientata B1.

关 键 词：牛膝 齐墩果酸 RP-HPLC 

分 类 号：TS207.4[轻工技术与工程—食品科学]