元宝枫叶多酚类物质的提取及对脂肪酸合酶的抑制研究  

Study on Extraction of Polyphenols from the Leaves of Acer truncatum and Their Inhibitory Abilities on Fatty Acid Synthase

作　　者：叶燕彬[1] 杨帅[1] 郜文[2] 张枫[1] 郭建强[2] 张英侠[1] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100069 [2]首都医科大学药品食品安全评价中心,北京100069

出　　处：《中药材》2009年第2期283-286,共4页Journal of Chinese Medicinal Materials

基　　金：北京市教委科技成果转化与产业化项目(PXM2007-014226-050086);北京市属市管高等学校"人才强教"资助项目(PXM2008-014226-053680)

摘　　要：目的:研究以乙醇为溶剂从元宝枫叶中提取抑制脂肪酸合酶的多酚类物质的工艺条件。方法:采用L16(45)正交试验,以提取时间、温度、溶剂组成和固液比为考察因素,以多酚的提取率以及对脂肪酸合酶的抑制率为检测指标,经过综合加权评分后得到优化提取工艺。结果:优选的提取工艺是乙醇体积分数为60%,固液比1g∶30mL,在50°C条件下,搅拌提取1.5h。结论:多酚类物质对脂肪酸合酶有抑制作用,从元宝枫叶中提取的某些多酚类物质具有相对较强的抑制作用。Objective ： To optimize a technical process for extracting the polyphenols that can inhibit fatty acid synthase by ethanol from the leaves of Acer truncatum. Methods：The extracting time, extracting temperature, extracting solvent and the ratio of solvent to raw material were studied by L16 （4^5） orthogonal experiments. The extraction rate of polyphenols and the inhibitory efficiency on the fatty acid synthase were utilized as evaluating criteria, and the optimum conditions for the extraction were obtained through summing up the above factors. Results： The efficient technological conditions were as follows： the concentration of ethanol was 60% （v/v）, the ratio of solvent to raw material was lg： 30mL; and the stirring time was 1.5h at 50℃. Conclusion： The polyphenols that extracted from the leaves of Acer truncatum have inhibitory activity against fatty acid synthase and some of them have stronger inhibitory effect.

关 键 词：元宝枫 多酚 脂肪酸合酶 正交试验 

分 类 号：R284.2[医药卫生—中药学]