刚地弓形虫NT株P30基因的克隆与表达  

Cloning and Expression of P30 Gene in NT Strain of Toxoplasma gondii

作　　者：张理航[1,2] 刘茂军[1] 吕芳[1] 李贺侠[1] 吴叙苏[1] 冯志新[1] 邵国青[1] 

机构地区：[1]江苏省农业科学院兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京210014 [2]金陵科技学院,江苏南京211169

出　　处：《江苏农业学报》2009年第1期147-150,共4页Jiangsu Journal of Agricultural Sciences

基　　金：农业部公益性行业(农业)科研专项(200803017)

摘　　要：参考Genbank登录的刚地弓形虫(Toxoplasma gondii)P30全基因序列,设计合成1对引物,通过PCR在刚地弓形虫的基因组DNA中扩增出P30基因片段,然后构建重组表达质粒pET-32 a(+)/P30,用IPTG诱导,在表达菌BL21(DE3)表达出分子量为4.83×104的融合蛋白,经W estern-b lotting鉴定目的蛋白具有良好的免疫学活性,为刚地弓形虫疫苗的制备及猪弓形虫抗体ELISA检测方法的建立奠定了基础。According to the sequence of Toxoplasma gondii P30 reported in GenBank, two primers were designed and synthesized, which were used to amplify partial fragments of P30 gene from the genomic DNA of T. gondii NT strain, then the recombinant pET-32 a（ ＋ ）/P30 vector including P30 gene of T. gondii was constructed, the fusion protein about 4. 83 × 10^4 was expressed in BL21 （ DE3 ） induced by IPTG. The result of Western-blotting proved that the fusion protein had good immunogenicity, which does the preparation for the development of T. gondii vaccine and the ELISA method of detecting the T. gondii antibody.

关 键 词：刚地弓形虫 P30基因 克隆 表达 

分 类 号：S852.729[农业科学—基础兽医学]