假单胞菌酶法转化DL-ATC合成L-半胱氨酸  

Progress on Enzymatic Synthesis of L-Cysteine from DL-ATC by Pseudomonas sp.

作　　者：吴敏[1] 陈蔚青[2] 王普[1] 何军邀[1] 

机构地区：[1]浙江工业大学生物制药研究所,浙江杭州310032 [2]浙江树人大学生物与环境学院,浙江杭州310015

出　　处：《氨基酸和生物资源》2009年第1期58-63,共6页Amino Acids & Biotic Resources

基　　金：浙江省科技厅重大项目资助(No.2006C13001);浙江省重中之重学科开放基金资助

摘　　要：采用微生物酶转化法制备L-半胱氨酸具有周期短、成本低、区域和立体选择性强、反应条件容易控制、环境友好等特点,与传统的毛发水解以及化学合成工艺相比显示出明显的优越性。本文从假单胞菌产酶条件和酶学性质、DL-ATC生物转化途径、固定化细胞转化工艺、基因工程菌的研究、以及L-半胱氨酸脱巯基酶的研究等5个方面介绍了国内外关于生物转化DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-ATC)合成L-半胱氨酸的研究进展。The microbial transformation method for L- cysteine production shows evident advantages, because of its short cycle time, low cost, high regio and stereoselectivity, easy control of reaction condition, and environment - friendly. Recently, studies on the bioeonversion of DL - 2 - amino - △^2- thiazoline - 4 - carboxylie acid （ DL - ATC） to L - cysteine by intracellular enzymes were reported. The research progresses on L - cysteine production by microbial bioeonversion, especially pseudomonas sp. , or its crude enzyme extract are summarized. The applications of immobilization technology in the biotransformation of DL -ATC to L -cysteine are introduced. The genetically engineered bacteria and the study progresses of L - cysteine desulfhydrase were also discussed.

关 键 词：假单胞菌 DL—ATC L-半胱氨酸 酶法转化 基因工程技术 L-半胱氨酸脱巯基酶 

分 类 号：Q939.9[生物学—微生物学]