膀胱癌D17S5位点VNTR多态性研究  

D17S5 Gene VNTR Loci Polymorphic in Bladder Cancer

作　　者：王德育[1] 袁春伟[1] 

机构地区：[1]东南大学国家教委分子与生物分子电子学实验室

出　　处：《东南大学学报（自然科学版）》1998年第2期151-154,共4页Journal of Southeast University：Natural Science Edition

摘　　要：膀胱癌Ｄ１７Ｓ５位点ＶＮＴＲ多态性研究王德育袁春伟（东南大学国家教委分子与生物分子电子学实验室，南京２１００９６）可变数目串联重复序列（ＶａｒｉａｂｌｅＮｕｍｂｅｒＴａｎｄｅｍＲｅｐｅａｔｕｎｉｔｓ，ＶＮＴＲｓ）是广泛存在于人类基因组中的ＤＮＡ片段...Variable number of tandem reperat units (VNTR s) can be isolated from every human chrosome. it is G rich sequence except Apo protein VNTR family. Its motif G -TGGG is almost invariant. Because its motif is the same as the χ sequence of phage λ 5′ GCT GGT GG 3′, which is hot spot of recombination, VNTR may have important function in genetic recombination. pYNZ 22 VNTR s mapping 17th chromosome, link p53 gene, which is an important anti cancer gene, consisting of a 70 bp reperat unit different in copy number. The core sequence is 5′ TGG AGT CTC TGG GTG TGG TGC GTC AGA GT 3'. pYNZ22 VNTR is related to Miller Dieker syndrome. Using polymerase chain reaction, following high solution PAGE technique, 26 case TCC pYNZ 22 VNTR are amplified. We find there are 14 allele in tumor, and their length are between 168 bp and 868 bp and frequency between 0 019 2-0 230 7. Compared with normal controls, the frequencies of allele is the difference significant ( P <0 001). The distribution of the allele frequency does not fit Hardy Weiberg balance ( P <0 001). The frequency of hetreogygosity in tumor organism(61 54%) is less than that in normal organism (83 85%), P =0 034. We think that the change of pens 22 VNTR frequency may be the result of deletion of some frequency.

关 键 词：膀胱癌 D17S5基因 VNTR位点 多态性 

分 类 号：R737.14[医药卫生—肿瘤]