靶向H-ras基因的小干扰RNA对卵巢癌SKOV3细胞增殖的抑制作用  

Inhibitory effect of small interference RNA targeting H-ras gene in ovarian cancer line SKOV3

作　　者：王萍玲[1] 胡丽娜[2] 

机构地区：[1]贵州省人民医院妇科,贵州贵阳55000 [2]四川大学华西二院妇产科

出　　处：《中国妇幼保健》2009年第5期664-667,共4页Maternal and Child Health Care of China

摘　　要：目的:探讨脂质体介导的靶向H-ras基因的小发夹状RNA重组质粒对人卵巢癌细胞株SKOV3细胞增殖的抑制作用。方法:实验分4组:正常对照组(仅予脂质体处理)、转染psh RNA/H-ras处理24h组、48h组及72h组。分别于转染后不同时间进行MTT实验、软琼脂克隆形成实验、细胞周期检测和AO/EB双染实验,观察各组细胞增殖、凋亡及细胞周期的变化。结果:FCM显示转染psh RNA/H-ras 24h及48h后的SKOV3细胞均出现细胞周期的抑制,48h后SKOV3细胞有显著的凋亡发生;转染psh RNA/H-ras1和pshRNA/H-ras2对SKOV3细胞克隆形成的抑制率分别为51.5%(P<0.05)和63.3%(P<0.01);MTT检测转染psh RNA/H-ras1和psh RNA/H-ras 272h后对SKOV3细胞增殖抑制率分别为62.5%和64.5%。AO/EB实验显示48h细胞的凋亡率为29.3%(P<0.05)。结论:重组质粒psh RNA/H-ras在人卵巢癌SKOV3细胞中有明显抑制细胞增殖的作用,并介导肿瘤细胞的凋亡。Objective： To explore the cells proliferation effect after transfected pshRNA/H -ras, discuss the rote of H -tas gene in the development of ovarian cancer. Methods： 4 groups were included in the study： control group, 24 hours experimental group, 48 hours experimental group and 72 hours experimental group after transfected pshRNA/H - ras. The influence on proliferation and apoptosis in 4 groups were investigated by MTT assay, cloning test, flow cytometry and AO/EB respectively. Results ： The inhibition effect of cell cycle after transfected pshRNA/H - ras 24 hours and 48 hours in SKOV3 strains was detected by FCM. The suppression rates of cloning efficiency were 51.5% （P 〈 0.05 ） and 63.3% （P 〈 0. 01 ） after transfectd pshRNA/H - rasl and pshRNA/H - ras2 in SKOV3, respectively. The apoptosis rate was 29.3% （P 〈0. 05） in AO/EB experiments. The suppression rates of cell proliferation were 62. 5% and 64. 5% after trans- fected pshRNA/H - rasl and pshRNA/H - ras2 72 hours by MTT in SKOV3 respectively. Conclusion ： The short hairpin RNA of H - ras can sunnress cells proliferation and enhance the anontosis of tumor cells in SKOV3 efficientlv.

关 键 词：RNA干扰 pshRNA/H—ras 卵巢癌 增殖 凋亡 

分 类 号：R73-754[医药卫生—肿瘤]