腺病毒携带凋亡素和内皮抑素双基因对小鼠肝癌细胞增殖及凋亡的影响  

Effect of adenovirus vector carrying apoptin and endostatin genes on the proliferation and apoptosis of liver cancer cell in mice

作　　者：吴本华 黄庆娟 朱惠明 

机构地区：[1]暨南大学第二附属医院深圳市人民医院,广东深圳518020

出　　处：《山东医药》2009年第7期1-3,共3页Shandong Medical Journal

基　　金：广东省卫生厅医学科研基金资助项目(A2006579)

摘　　要：目的探讨肝癌基因治疗的新方法。方法建立C57 BL/6j小鼠皮下种植性肝癌模型,随机分为A、B、C、D组各8只。A组注射腺病毒携带凋亡素和内皮抑素双基因,B组注射腺病毒携带凋亡素单基因,C组注射腺病毒携带绿色荧光蛋白基因,D组注射生理盐水。治疗后连续12 d监测各组肿瘤体积,计算抑瘤率,RT-PCR法检测肿瘤组织中凋亡素、内皮抑素mRNA表达;TUNEL染色法检测肿瘤组织的原位凋亡并计算凋亡指数。结果治疗后第7天A组肿瘤体积明显小于其他各组,抑瘤率明显高于其他各组(P均<0.05);A组肿瘤组织中有凋亡素和内皮抑素mRNA表达,B组肿瘤组织中有凋亡素mRNA表达;A组凋亡指数明显高于其他各组,P<0.05。结论腺病毒携带凋亡素和内皮抑素双基因能显著抑制小鼠肝癌细胞生长并能诱导其凋亡;该病毒有望用于肝癌的基因治疗。Objective To investigate the new method for the gene therapy of liver cancer. Methods Model of subcutaneous liver cancer in mice were established and randomly divided into A,B,C and D groups. Group A were injected with adenovirus vector carrying apoptin and endostain genes, group B and C were injected with adenovirus carrying apoptin and GFP gene, while group D were injected with normal sailine. The volume of the tumors was observed for 12 days after treatment. The expression of apoptin and endostatin genes was detected by RT-PCR and the apoptosis of tumor ceU in vivo was detected by TUNEL. Results Compared with the other three groups, the volume of tumors in group A were significantly smaller（ P 〈0.05 ） , while the inhibition and the apoptotic index were higher. The apoptin gene was expressed in group A and B, while the endostatin gene was only expressed in group A. Conclusion The adenovirus vector carrying apoptin and endostatin genes can notably inhibit the growth of the subcutaneous liver cancer and induce the apoptosis in mice.

关 键 词：凋亡素基因 内皮抑素基因 腺病毒载体 肝癌细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]