苹果IRAP技术体系的建立及优化  

Establishment and optimization of the inter -retrotransposon amplified polymorphism(IRAP) molecular marker system in apple

作　　者：贾怀志[1] 刘艳红[1] 渠慎春[1] 高志红[1] 王昆[2] 章镇[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《果树学报》2009年第2期254-257,共4页Journal of Fruit Science

基　　金：国家自然科学基金(No.30671438)

摘　　要：通过苹果逆转座子长末端重复序列(LTR)保守区域设计引物,对影响苹果IRAP反应的重要因素进行了研究,建立并优化了苹果IRAP分子标记技术体系。优化的苹果IRAP分子标记体系为:25μL反应体系中,模板DNA50ng、Mg2+2.5mmol·L-1、dNTPs0.2mmol·L-1、10×buffer2.5mmol·L-1、TaqDNA聚合酶1.0U、引物0.4μmol·L-1。该体系在所试苹果品种中均获得了较理想的扩增效果,并能鉴定部分芽变品种。IRAP primers were designed based on Long Terminal Repeat （LTR） reserve region of retrotransposon in apple. Several important reaction factors of IRAP were studied in order to establish and optimize IRAP system in apple .The final optimal system was established as follows： template DNA 50 ng, Mg^2＋ 2.5 mmol· L^-1 ,dNTP 0.2 mmol· L^-1, 10xbuffer 2.5 mmol· L^-1, Taq DNA polymerase 1.0 U, primer 0.4 mmol· L^-1 in 25 μL reaction system. Ideal amplification results were obtained by using such IRAP system in tested apple cuhivars, especially in identifying the bud mutation.

关 键 词：苹果 逆转座子 IRAP 技术体系 

分 类 号：S661.1[农业科学—果树学]