GPR48单克隆抗体的制备及其在甲状腺癌中的表达  

Preparation of Monoclonal Antibody against GPR48 and Its Expression in Human Thyroid Cancer

作　　者：关海霞[1] 宝荣[2] 王翠芳[3] 高芸[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,内分泌研究所,沈阳110001 [2]重庆市第九人民医院病理科,重庆400000 [3]沈阳市奉天医院病理科,沈阳110024

出　　处：《中国医科大学学报》2009年第1期19-21,25,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30740087)

摘　　要：目的制作G蛋白偶联受体48(GPR48)的单克隆抗体并研究其在甲状腺癌中的表达。方法利用细胞融合法制作单克隆抗体,蛋白免疫电泳法检测单克隆抗体。免疫组化法研究GPR48蛋白在90例甲状腺癌及35例良性甲状腺组织中的表达情况。结果成功制备了GPR48的特异性单克隆抗体。良性甲状腺疾病组织和癌对侧的甲状腺组织中几乎没有GPR48的表达。甲状腺癌组织中GPR48表达阳性率为61.1%(55/90)。未分化型甲状腺癌和分化型甲状腺癌中GPR48的表达阳性率分别为80.0%和55.7%(P=0.09),伴有淋巴结转移和不伴有转移的分化型甲状腺癌中GPR48的表达阳性率分别为77.3%和45.8%(P=0.03)。结论GPR48抗体可用于相应蛋白的特异性检测,GPR48可作为提示肿瘤分化和淋巴结转移的新标记物。Objective To prepare the monoclonal antibody against GPR48 and detect its expression in thyroid cancer. Methods The monoclonal antibody against GPR48 was prepared by cell fusion and identified by Western blot. The expression of GPR48 was determined in thyroid cancer tissues （n=90） and benign thyroid tissues （n=35） by immunohistochemical staining. Results The monoclonal antibody of GPR48 was successfully prepared. GPR48 protein was expressed in 61.1% （55/90） of the thyroid cancer tissues. The positive expression rate of GPR48 was higher in anaplastic thyroid cancer than in differentiated thyroid cancer including papillary thyroid cancer and follicular thyroid cancer （80.0% vs 55.7% ,P = 0.09）. In cases of differentiated thyroid cancer,the positive expression rate of GPR48 in cases with lymph node metastasis was significantly higher than that in cases without lymph node metastasis （77.3% vs 45.8%,P = 0.03）. Conclusion The monoclonal antibody against GPR48 can detect GPR48 protein specifically. GPR48 may be used as a new marker for predicting tumor cell differentiation and lymph node metastasis.

关 键 词：G蛋白偶联受体48 单克隆抗体 甲状腺癌 

分 类 号：R736.1[医药卫生—肿瘤]