成人喉乳头状瘤的基因芯片研究  

Gene Expression Profiling of Adult-onset Laryngeal Papilloma

作　　者：白伟良[1] 宋岩[1] 高红[2] 

机构地区：[1]中国医科大学附属盛京医院1.耳鼻咽喉科 [2]卫生部小儿先天畸形重点实验室,沈阳110004

出　　处：《中国医科大学学报》2009年第1期22-25,共4页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20051038);沈阳市社会发展基金资助项目(041101)

摘　　要：目的了解肿瘤相关基因在喉乳头状瘤中的表达情况,探讨其与喉癌组织中的基因表达差异。方法提取喉乳头状瘤、喉鳞癌及癌旁正常组织的总RNA,采用Ampo Labeling-LPR方法,将合成的探针与肿瘤相关基因SuperArray Q芯片膜进行杂交,将X线片感光点转化成TIFF图形文件,使用ScanAlyze和GEArray Analyzer软件进行资料分析。结果与正常喉黏膜组织相比,成人喉乳头状瘤存在细胞间黏附分子5,组织蛋白酶基因D,LIM激活酶基因,RhoC,CD31等11个基因表达上调,占11.5%(11/96),仅1个基因下调,占1.0%(1/96),与喉乳头状瘤相比,喉癌组织出现N-羟乙酰神经氨酸转移酶V基因,骨桥蛋白基因,组织蛋白酶基因L,金属基质蛋白酶基因15,胰岛素样生长因子II,CD44等6个基因表达上调,占6.3%(6/96),下调基因仅1个,占1.0%(1/96)。结论喉乳头状瘤基因表达谱的特点是在所观察的与癌相关96个基因中,基因表达介于癌旁正常组织与喉癌肿瘤组织之间,研究喉乳头状瘤的基因表达谱特点为我们探索喉癌发生、发展提供了有益的线索。Objective To explore the differential gene expression profiling between adult-onset laryngeal papilloma and laryngeal squamous cell carcinoma. Methods Total RNA was purified from 1 case of adult-onset laryngeal papilloma and 1 case of laryngeal squamous cell carcinoma and its adjacent normal laryngeal tissues, cDNA probe was synthesized with AmpoLabeling-LPR method and then hybridized with Q Series Human Tumor Metastasis Gene Array （SABiosciences Company, US ）, followed by chemiluminescent detection. The data were analyzed with SeanAlyze and GEArray Analyzer. Results Compared with normal laryngeal tissues, 11.5%（ 11/96）of the genes, such as ICAM- 5, CD31, Cathepsin D, LIM Kinase, and Rhoct, were up-regulated and only 1.0%（ 1/96 ）of the genes down-regulated in the adult-onset laryn- geal papilloma. Compared with the adult-onset laryngeal papilloma, 6.3%（6/96）of the genes,such as MGAT5, Osteopontin, CD44, Cathepsin L, matrix metalloproteinase 15 ,and IGF-Ⅱ,were up-regulated and only 1.0%（ 1/96 ）of the genes down-regulated in the laryngeal squamous cell carcinoma. Conclusion Of the 96 cancer-associated genes, the expression profiling of adult-onset laryngeal papilloma is between that of normal laryngeal tissue and laryngeal squamous cell carcinoma,which may provide some clues to study the occurrence and development of laryngeal carcinoma.

关 键 词：喉乳头状瘤 喉癌 基因芯片 

分 类 号：R739.65[医药卫生—肿瘤]