熊果酸和环氧化酶-2抑制剂对胃癌细胞增殖的影响  

Effects of ursolic acid and meloxicam on proliferation of gastric cancer cells

作　　者：李剑萍[1] 邵耘[1] 陈国胜[1] 何晓璞[1] 刘翠霞[1] 许海尘[1] 周苏明[1] 孙为豪[1] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210029

出　　处：《江苏医药》2009年第3期305-307,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅"兴卫工程"医学重点人才基金(RC2007046)

摘　　要：目的探讨熊果酸和美洛昔康对人胃癌细胞株SGC-7901增殖的调控作用。方法SGC-7901细胞接种于含10%胎牛血清的RPMI-1640培养液中,常规培养24h后加熊果酸或美洛昔康,药物终浓度均为10、20、40、80μmol/L,分别培养12、24和48h,四甲基偶氮唑盐(MTT)比色分析细胞增殖,Westernblot检测环氧化酶-2(COX-2)表达。结果熊果酸和美洛昔康两者均剂量和时间依赖性地抑制SGC-7901细胞增殖,熊果酸的抑制作用小于美洛昔康(P<0.05)。熊果酸和美洛昔康剂量依赖性地抑制SGC-7901细胞COX-2表达,熊果酸抑制COX-2表达的作用小于美洛昔康(P<0.05)。结论熊果酸和美洛昔康均抑制胃癌细胞增殖;其机制可能与COX-2表达下调有关。Objective To investigate the regulative effects of ursolic acid and meloxicam on the proliferation of human gastric cancer cell line SGC-7901. Methods SGC-7901 cells were seeded in RPMF1640 supplemented with 10% heat-inactivated fetal calf serum and routinely incubated for 24 h. After treatment with either ursolic acid or meloxicam both at a final concentration of 10,20,40 or 80 gmol/L for 12,24 and 48 h. The cell proliferation was determined using methyl thiazolyl tetrazolium （MTT） colorimetric assay and the expression of cyclooxygenase-2 （COX-2） protein level was measured with Western blot. Results Both ursolic acid and meloxicam significantly inhibited SGC-7901 cell proliferation in a dose-and time-dependent manner. The inhibitory rate in the cells treated witla ursolic acid was significantly lower than that treated with meloxicam （P〈0. 05）. Ursolic acid and mcloxicam dose-dependently inhibited COX-2 expression in SCX2-7901 cells. The inhibitory effect of ursolic acid on the expression of COX-2 was significantly lower than that of meloxicam （P〈0.05）. Conclusion Both ursolic acid and meloxicam inhibit the proliferation of gastric cancer cells, which may be related to down-regulation of COX-2 expression.

关 键 词：胃癌 熊果酸 美洛昔康 环氧化酶-2 

分 类 号：R735[医药卫生—肿瘤]