CT灌注成像对大鼠急性脑缺血及再灌注模型的评价  

Evaluating rat model of acute cerebral ischemia-reperfusion by computed tomography perfusion imaging

作　　者：马秀华[1] 吕富荣[1] 吕发金[1] 肖智博[1] 黄铀新[1] 李新友[1] 杨柳[1] 潘静[1] 

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016

出　　处：《第三军医大学学报》2009年第6期506-509,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(2007BB5292);重庆医科大学创新基金(CX200501)~~

摘　　要：目的在活体状态下应用CT灌注成像(CT perfusion imaging,CTPI)技术,评价大鼠急性脑缺血及再灌注模型的可靠性及准确性。方法用改良线栓法制作大鼠急性脑缺血及再灌注模型,分别行CTPI检查、2,3,5-三苯基氯化四氮唑(TTC)染色及HE染色。结果①每组CTPI各时间点脑血流量(cerebral blood flow,CBF)图核心区与脑血容量(cere-bral blood volume,CBV)图低灌注区体积比较无统计学差异(P>0.05),两者所示体积与TTC染色梗死区体积比较无统计学差异(P>0.05),与平均通过时间(mean transit time,MTT)图缺血区体积比较有统计学差异(P<0.05)。MTT缺血区体积与TTC染色梗死区体积比较有统计学差异(P<0.05)。②CTPI各参数图与TTC染色梗死体积间均呈正相关关系,其中CBV相关性最好(r=0.74)。③梗死区CBF、CBV较对侧均明显减低,MTT延长;缺血半暗带区(Ischemic penumbra,IP)CBF较对侧明显减低,CBV较对侧正常或升高,MTT轻度延长;周围相对正常区CBF、CBV及MTT较对侧未见明显改变。④取梗死区脑组织做HE染色,结果显示以神经细胞死亡表现为主;取IP区脑组织做HE染色,显示以神经细胞变性损伤为主。结论CTPI可在活体状态下快速、准确、无创地评价脑缺血再灌注动物模型的脑部血流动力学改变,显示脑梗死区及缺血半暗带区的部位、大小、程度,对超急性期脑梗死治疗及复查具有较强的临床应用价值。Objective To evaluate the reliability and accuracy of rat model of acute cerebral ischemiareperfusion in living condition by using CT perfusion imaging （CTPI） technology. Methods A stable and reversible acute cerebral ischemia-reperfusion model with unilateral middle cerebral occlusion was established in Wistar rats and evaluated by CTPI, TTC staining and HE staining. There were 2 groups： ischemia group and ischemia ＋ reperfusion group. Results （1）Cerebral blood flow （CBF） core areas and cerebral blood volume （CBV） of low volume infusion areas on CTPI showed no significant difference at various time points and between the 2 groups （ P 〉 0. 05 ）, and the results obtained from CTPI were concordant with those from TTC staining for infarction zone （ P 〉 0. 05 ） , but significantly different with the isehemic volume of average transit time （MTT） （P 〈 0. 05 ）. There were significant differences between MTT ischemic volume and infarction zone volume by TTC staining （ P 〈 0.05 ）. （2）There was correlation between CTPI parameters and infarction volume by TTC staining, and CBV relevance was the best （r = 0. 74）. （3）CBF and CBV in infarction zone were significantly lower than those in contralateral control zone, and MTT extended; CBF in ischemic penumbra （IP） zone was significantly lower than that in contralateral control zone, while CBV was normal or higher, and MTT extended slightly; CBF, CBV and MTT in relatively normal zone had no obvious changes than these on contralateral sides. （4）The brain tissue with the matched decrease in CBF and CBV showed the performance-based nerve cell death in HE staining, and the brain tissue with CBF decreased and CBV normal showed the performance-basednerve cell degeneration. Conclusion CTPI as a rapid, accurate, non-invasive method can evaluate the brain homodynamics of cerebral ischemia-reperfusion animal model in living state, and can display the site, size, extent of ischemic region during cerebral infarction, and has

关 键 词：CT灌注成像 急性脑缺血 脑部血流动力学 半暗带 大鼠 

分 类 号：R-332[医药卫生] R743.31