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作 者:吴静[1,2] 强占荣[2] 林香春[1] 杨国栋[2] 周永宁[2] 王爱勤[3] 薛群基[3]
机构地区:[1]北京世纪坛医院,北京大学第九临床医学院消化科,北京100038 [2]兰州大学第一医院消化科,兰州730000 [3]中国科学院兰州化学物理研究所,兰州730000
出 处:《第三军医大学学报》2009年第6期545-547,共3页Journal of Third Military Medical University
基 金:中国科学院西部之光项目(CX805)~~
摘 要:目的探讨D-氨基葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(COPADG)对人食管癌Eca-109细胞凋亡的影响及其作用机制。方法体外培养Eca-109细胞;倒置显微镜下观察细胞形态学变化;流式细胞术检测COPADG作用后Eca-109细胞内半胱氨酸酶3(caspase-3)活化程度及bcl-2蛋白表达的变化;Annexin V/PI染色结合流式细胞术检测COPADG作用后Eca-109细胞凋亡率。结果COPADG作用显著增加Eca-109细胞凋亡率,且Eca-109细胞内caspase-3活性和bcl-2表达平均荧光强度明显增高。结论COPADG能显著诱导Eca-109细胞凋亡发生,并使Eca-109细胞内caspase-3显著活化以及bcl-2蛋白表达下调,并可能通过该机制诱导细胞凋亡的发生。Objective To observe the effect of D-glucosamine 2-((3-carboxy-1-oxopropyl) amino)-2-deoxy-D-glucose (COPADG) on apoptosis and caspase-3 activation and bcl-2 protein expression in human esophageal cancer Eca-109 cells and discuss the possible mechanisms. Methods Eca-109 cells were cultured in vitro, then exposured to 0.03 mmol/L COPADG for different time periods. The cell morphological changes were observed by inverted phase contrast microscopy. Caspase-3 activity and bcl-2 expression were detected by flow eytometry, and apoptosis rate was analyzed by Annexin V/PI fluorescence staining together with flow cytometry. Results COPADG could significantly induce Eca-109 cells to apoptosis, and increase mean fluorescence intensity of caspase-3 and bcl-2 protein expressions in the cells. Conclusion COPADG induces the apoptosis of human esophageal cancer cell line Eca-109, as well as the activation of caspase-3 and the downregulated expression of bcl-2 protein. This may not an unique mechanism in COPADG-induced apoptosis.
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