腺病毒介导GRIM19对恶性胶质瘤CHG-5细胞增殖及分化的影响  

Effects of recombinant GRIM19 gene adenovirus on cell proliferation and differentiation of human glioma cell line CHG-5

作　　者：姚声涛[1] 陈佳琳[2] 黄轶[3,4] 郭川[1] 唐文渊[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016 [2]重庆医科大学基础医学院生物化学与分子,重庆400016 [3]附属儿童医院临床分子医学中心,重庆400014 [4]贵州省遵义医院儿科,贵州遵义563002

出　　处：《第三军医大学学报》2009年第6期548-551,共4页Journal of Third Military Medical University

摘　　要：目的观察增强外源性GRIM19(genes associated with retinoid-IFN-induced mortality 19)基因表达对恶性胶质瘤CHG-5细胞生物学特性的影响,初步探讨其机制。方法利用已成功构建的Ad-GRIM19腺病毒感染CHG-5细胞,通过形态学、细胞生长曲线、软琼脂克隆形成等观察增强GRIM19基因表达后CHG-5细胞形态、增殖和体外成瘤能力的变化,台盼蓝染色法检测细胞活力,Real-timePCR及Western blot检测STAT3与PCNA基因表达。结果Ad-GRIM19在体外感染CHG-5细胞后,明显改变CHG-5细胞生长形态,细胞增殖及体外成瘤减低,STAT3、PCNA基因表达下调。结论GRIM19过表达可显著抑制CHG-5细胞恶性表型,提示GRIM19基因在调控胶质瘤细胞分化程度及恶性生物学行为方面具有重要作用。Objective To observe the effects of enhanced genes associated with retinoid-IFN-induced mortality 19 （GRIM19） expression on biological features of human malignant glioma cells in vitro. Methods Human glioma cell line CHG-5 were infected with Ad-GRIM19 and Ad-GFP （control）. The infected and control glioma cells were analyzed for their morphological alterations and cell growth features by using WST-8, soft agar cloning-formation and other methods. Real-time PCR and Western blotting were employed to detect the mRNA and protein expressions of STAT3 and PCNA. Results Compared with control cells, CHG-5 cells transfected with Ad-GRIM19 demonstrated profound morphological alterations and marked cellular atypia. Cell proliferation rate and their tumorigenecity in vitro were also decreased. The mRNA and protein expressions of STAT3 and PCNA were downregulated. Conclusion Overexpression of GRIM19 gene has affected CHG-5 glioma cell morphology and decreased their growth and tumorigenecity, suggesting GRIM19 gene might play a key role in determining differentiation and certain malignant biological features of glioma cells.

关 键 词：GRIM19 腺病毒 胶质瘤 CHG-5细胞 

分 类 号：R394.2[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]