PEDF对大鼠穿透性角膜移植片VEGF影响的实验研究  

Experimental study of the effects of PEDF on penetrating corneal graft of VEGF in rats

作　　者：步建平[1] 周善璧[1] 程琳[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,重庆400016

出　　处：《重庆医科大学学报》2009年第2期152-156,共5页Journal of Chongqing Medical University

基　　金：重庆市卫生局资助项目(03-2-097)

摘　　要：目的:探讨局部应用色素上皮细胞衍生因子(Pigment epithelium-derived factor,PEDF)滴眼液抑制大鼠穿透性角膜移植术后植片中VEGF(Vascular endothelial growth factor,VEGF)的表达及作用机制。方法:建立60只SD大鼠(受体)和30只Wista(r供体)大鼠同种异体穿透性角膜移植模型,并随机分成实验组(10nmol/LPEDF滴眼液)和实验对照组(赋形剂滴眼液)各30只;另30只SD大鼠自体穿透性角膜移植模型(赋形剂滴眼液),作为空白对照组,每只动物均行右眼手术左眼为正常对照。自术后第1d起在显微镜下进行临床观察,每日1次。于术后1、2、3、4、8周5个时间点,每组随机处死6只动物,以免疫组织化学方法检测角膜移植片PEDF和VEGF的表达水平;透射电镜观察超微结构变化。结果:实验组在术后2、3、4、8周VEGF的表达水平显著低于实验对照组(P<0.05或P<0.01)。结论:局部应用PEDF滴眼液可以降低穿透性角膜移植植片中VEGF的表达,延长大鼠穿透性角膜移植片的存活时间。Objectives： To evaluate the effects of PEDF eyedrops on the corneal allograft rejection by topical installition in the rats model of penetrating keratoplasty, and to investigate its possible mechansism and its correlation factor. Methods： Penetrating keratoplasty （PKP） was proformed orthotopically in wistar rats and SD rat recipients. 60 SD rats were randomly assigned to two groups ; experimental group and experimental control group after PKP. 30 SD rats underwent auto penetrating keratoplasty（blank control group ）. The experimental group was treated with PEDF eyedrops, while the blank control group and experimental control group were treated with escipient eyedrops. All animals＇ surrival time was recored and compared by the biomicroscope examination. 6 animals in three groups were killed respectively at the 1 st week, 2nd week, 3rd week, 4th week and 5th week postoperatively. Corneal uhrastuctural morphology was observed by TEM. PEDF and VEGF expression was examined by immunohistochemistry. Results： Expression level of VEGF of experimental control group was higher than that of experimental group（P〈0.05 or P〈0.01 ）. Conclusion： PEDF eyedrops can depress expression of VEGF in corneal allografts. Topical application of PEDF eyedrops can prolong suivival time of the allografts.

关 键 词：色素上皮细胞衍生因子 免疫排斥反应 内皮细胞生长因子 角膜新生血管 

分 类 号：R772.2[医药卫生—眼科]