脑内移植间充质干细胞治疗大鼠帕金森病模型的研究  

Intracerebral Transplantation of Rat Bone Marrow-derived Mesenchymal Stem Cells for Treatment of 6-OHDA Model of Parkinson's Disease

作　　者：付文玉[1] 邢海霞[1,2] 王晓萃[1] 吕娥[3] 王玉良[4] 郑志娟[1] 庄文欣[1] 

机构地区：[1]潍坊医学院组织学与胚胎学教研室,山东潍坊261042 [2]温州医学院附属医院病理科 [3]组潍坊医学院织学与胚胎学教研室,山东潍坊261042 [4]潍坊医学院生理学教研室,山东潍坊261042

出　　处：《解剖科学进展》2009年第1期32-36,共5页Progress of Anatomical Sciences

基　　金：山东省自然科学基金资助项目(Y2003C01);山东省"泰山学者"建设工程专项经费资助;潍坊医学院博士启动基金

摘　　要：目的研究大鼠骨髓间充质干细胞(MSCs)脑内移植治疗帕金森病(PD)大鼠的可行性。方法贴壁培养法分离、培养大鼠骨髓MSCs。将BrdU标记的第3代MSCs移植到PD大鼠纹状体内,移植后1、2和4周检测PD大鼠的行为学变化,应用BrdU免疫荧光观察移植细胞在脑内的存活情况,应用免疫组化法检测酪氨酸羟化酶(TH)在移植细胞及黑质的表达。结果移植细胞后1周、2周和4周PD大鼠与移植前相比行为学有改善,旋转圈数明显减少(P<0.05);PD大鼠损毁侧和健侧黑质内TH阳性细胞数之比随移植时间的延长而增加,但未发现移植细胞呈现TH阳性表达。细胞移植后1周、2周和4周检测均可见纹状体内BrdU阳性细胞散在分布。结论植入脑内的MSCs能够生存和迁移,MSCs脑内移植对PD大鼠有一定的治疗作用。Objective To investigate the possibilities of the intracerebral transplantation of rat bone marrowderived mesenchymal stem cells （ MSCs ） for treatment of 6-OHDA model of Parkinson＇s disease（PD）. Methods The rat MSCs from bone marrow were cultured by adhering to the culture plastic method. The BrdU labeled and pured MSCs of the third generation were transplanted into the corpus striatum of the 6-OHDA injected side of PD model rats. 1, 2 and 4 weeks after transplantation, the ethological changes of PD rats were detected and the survival of the transplanted cells in the corpus striatum was examined by BrdU immunofluorescence and the tyrosine hydroxylase（TH） expression in substantia nigra and the transplanted cells in the corpus striatum was checked by immunohistochemistry. Results 1, 2 and 4 weeks after MSCs being transplanted into the corpus striatum of the 6-OHDA injected side of PD rats, the rotational behavior of PD rats was improved significantly（P 〈 0.05）, with more number of the rotational circle before MSCs transplanation than after MSCs transplanation. The ratio of TH positive cells in 6-OHDA injected side in comparison with normal side increased as the prolongation of MSCs transplantation in the suhstantia nigra compacta（SNc） of PD rats, but no TH positive cells were found in transplanted MSCs in SNc. The BrdU positive ceils were seen to be scattered in the corpus striatum 1, 2 and 4 weeks after MSCs transplantion. Conclusion The transplanted MSCs can survive and migrate in the PD rat brain and may have effect for the treatment of PD.

关 键 词：间充质干细胞 帕金森病 移植 骨髓 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]