肺癌中TSG101蛋白与Notch3受体表达的相关性  

The Relationship between TSG101 Protein and Notch3 Receptor in Lung Cancer

作　　者：常家辉[1] 陆平[2] 蔡存伟[3] 李菲[1] 钟志永[1] 张道荣[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,沈阳110001 [2]齐齐哈尔医学院附属五院大庆市龙南医院病理科,大庆163453 [3]辽宁省肿瘤医院病理科,沈阳110042

出　　处：《中国肺癌杂志》2009年第2期111-116,共6页Chinese Journal of Lung Cancer

摘　　要：背景与目的TSG101蛋白是内吞分选通路的重要因子,该通路的异常会减少果蝇中Notch受体的降解,导致此受体信号对细胞分化、发育调节的紊乱。在哺乳动物中,Notch受体家族中的3亚型与肺组织发育及肺癌发生密切相关。本实验的目的是初步探讨在人类肺癌中,TSG101蛋白与Notch3受体之间的表达是否也存在关联。方法分别采用Western blot和免疫组化SP法检测TSG101蛋白与Notch3受体在肺癌组织、细胞系中的表达,并应用抗体封闭TSG101蛋白功能后观察Notch3受体的表达变化。结果TSG101蛋白在肺癌中比正常肺组织表达减少,而Notch3受体在肺癌中比正常肺组织表达则增多,此变化均与肺癌的分化及淋巴结转移相关。当用抗体封闭肺癌细胞系中TSG101蛋白功能时,Notch3受体检测水平呈上升变化。结论肺癌中TSG101蛋白表达下调,与Notch3受体检测水平上升有相关性。Background and objective TSG101 protein is one of the key factors in Endosomal sorting pathway. The abnormality of this pathway could decrease degradation of Notch receptor which leads to disorder of cell differentiation and development in drosophila. In mammal, the Notch3 receptors correlate with lung tissue development and lung carcinogenesis. The purpose of this study is to explore the relationship between TSG101 protein and Notch3 receptor in human lung cancer. Methods Immunohistoehemical method （S-P method） and Western blot were applied to detect the expressions of TSG101 protein and Notch3 receptor in lung cancer tissues and cell lines, in comparison with the corresponding normal tissues and cell lines. Besides, the expression of Notch3 receptor was observed when TSG101 protein was blocked by its specific antibody. Results The TSG101 protein expressions in tumor tissues were significantly lower than those in the neighboring noncancerous tissue, with higher expressions of Notch3 receptor in tumor tissues, which were correlated with the cellular differentiation and lymph node metastases. The expression of Notch3 receptor increased after blockage of TSG101 protein by its specific antibody. Conclusion Down-regulation of TSG101 protein was correlated with the upregulation of Notch 3 receptor in lung cancer.

关 键 词：肺肿瘤 TSG101蛋白 Notch3受体 内吞分选 

分 类 号：R734.2[医药卫生—肿瘤]