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作 者:王新允[1] 袁玲[1] 郑海燕[1] 刘娟[1] 黄曦[1] 武力[1] 袁海洪[1] 赵剑斓[1]
机构地区:[1]天津医科大学病理教研室,天津医科大学总医院病理科,天津医科大学二附属医院病理科,天津300070
出 处:《中国肺癌杂志》2009年第2期131-134,共4页Chinese Journal of Lung Cancer
基 金:天津市科委自然科学基金重点项目(No.033804211)资助~~
摘 要:背景与目的脆性组氨酸三联体(fragile histidine triad,FHIT)是1996年由Ohta等克隆的候选抑癌基因,文献报道在多种与环境致癌物有关的恶性肿瘤中,常有FHIT的改变。本研究应用免疫组化方法检测FHIT蛋白在细胞芯片中的表达情况,进一步验证细胞芯片,为快速、简便、经济、规范地进行各项细胞学检查提供一种新方法。方法收集天津医科大学总医院、天津市第一中心医院及天津市胸科医院2005年5月-8月有肿瘤细胞的肺癌患者的胸水50例、非肿瘤患者胸水6例,运用自制的细胞芯片检测装置,制备112点细胞芯片。应用免疫组织化学方法检测细胞芯片中FHIT蛋白的表达,采用SPSS11.5统计软件进行数据处理。结果细胞芯片排列整齐,分布均匀,无掉片,符合观察需要。细胞芯片免疫组化染色结果与进行FHIT免疫组化染色的组织芯片中Ⅲ+Ⅳ期肺癌的检测结果进行比较,差异均无统计学意义(P>0.05)。结论细胞芯片具有操作简单,信息量大,节约试剂,减少误差,诊断快速的特点,在临床诊断、科学研究和流行病学筛选中具有广泛的应用前景。Background and objective Fragile histidine triad (FHIT) is a candidate tumor suppressor gene. Aberrance of FHIT has been observed in multiple carcinomas induced by environmental carcinogens, especially in lung cancer. In this study, the expression of FHIT protein in cell array was detected to further development of cell array and for a rapid, simple, and economical method. Methods The lung cancer samples were collected from the Tianjin Medical University General Hospital, the First Central Hospital and the Tianjin Chest Hospital from May to August in 2005. A total of 112 dots cell array were constructed in this study including the pleural fluid of 50 cases of lung cancer and 6 cases of normal, the cell array was done with the home-made cell array survey setting. Immunohistochemical stains were performed. Results All points rank in a good order without distortion in cell array. After IHC stains of FHIT, the number of cells did not decrease. The positive expression was totally the same to that from the tissue microarry. Conclusion Cell array is a rapid and high-throughput technique with high specificity, which could be broadly used in the clinical diagnosis and the screen of epidemilology.
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