富血小板血浆促进人牙髓细胞增殖的实验研究  

Effects of different concentration platelet rich plasma on human pulp cells proliferation

作　　者：唐凤勤[1] 邹德荣[1] 陆家瑜[1] 华丽[1] 曹春花[1] 

机构地区：[1]上海交通大学附属第六人民医院口腔科,上海200233

出　　处：《口腔医学》2009年第3期137-141,共5页Stomatology

摘　　要：目的探讨不同浓度富血小板血浆(Platelet rich plasma,PRP)对人牙髓细胞(human dental pulp cells,HDPCs)的增殖作用。方法两步离心法制备PRP,通过ELISA的方法测定PRP中两种主要生长因子血小板源性生长因子(PDGF-AB)和转化生长因子(TGF-β1)的浓度;用四唑盐比色法(MTT)观察5%、10%、20%PRP在2d、4d时对牙髓细胞的增殖作用,并探讨这种作用是否依赖于胎牛血清(FBS)的存在。结果所制备的PRP中血小板的浓度大于1000×109个/L,为全血中的4倍以上,经ELISA的方法测定PRP及贫血小板血浆(Platelet poor plasma,PPP)中PDGF-AB、TGF-β1的浓度分别增加4倍以上。MTT法测定不同浓度组的PRP对牙髓细胞均有增殖作用,以10%PRP增殖效应最明显,P值均<0.05;4d时PRP对细胞的增殖作用明显强于2d时,P值<0.05;10%PRP组较10%胎牛血清组增殖作用明显,P值<0.05。结论本实验制备的PRP含有较高浓度的PDGF-AB及TGF-β1,不同浓度的PRP均能有效促进牙髓细胞增殖,以10%PRP浓度增殖效应最明显;并且这种增殖作用并不依赖于胎牛血清的存在;随着时间延长,PRP对细胞增殖作用增加。Object To evaluate the effects of different concentration platelet rich plasma（PRP） on human dental pulp cells（HDPCs） proliferation. Methods We used two steps to obtain PRP from two volunteers. PDGF-AB and TGF-β1 were evaluated by enzyme-linked immunosorbent assay（ ELISA）. HDPCs were exposed to various concentrations of PRP（5 %, 10%, 20% ） and DMEM（ negative control）, respectively. After 2 days and 4 days, cell proliferation was evaluated using MTT assay. Results The concentrations of platelet in PRP were more than 1000 × 10^9/L and at least 4 times more than the baseline. TGF-β1 and PDGF-AB levels were high in activated PRP. The effects of various concentrations of PRP on cell proliferation were significantly greater than those of negative control（ P 〈 0.05）. The most obvious effects of proliferation were in 10% PRP group. The effects of proliferation on 4d were greater than those on 2d. And the effects were independent of the adding of fetal bovin serium. Conculusions The PRP contained high concentrations of PDGF-AB, TGF-β1. Various concentrations of PRP had obvious effects on human dental pulp cells proliferation.

关 键 词：牙髓细胞 富血小板血浆 生长因子 修复性牙本质 细胞增殖 

分 类 号：R781.31[医药卫生—口腔医学]