PCR和FISH技术在子宫颈淋巴瘤与淋巴瘤样病变诊断和鉴别诊断中的作用  

Role of PCR and FISH in diagnosis and differential diagnosis of primary lymphoma and lymphoma- like lesions of the uterine cervix

作　　者：刘翠苓[1] 李敏[1] 黄欣[1] 董格红[1] 张燕[1] 高子芬[1] 

机构地区：[1]北京大学基础医学院病理学系,100191

出　　处：《白血病．淋巴瘤》2009年第3期139-141,共3页Journal of Leukemia & Lymphoma

基　　金：基金项目：卫生行业科研专项项目（200802011） 致谢 感谢英国剑桥大学病理学系杜明清教授实验室叶洪涛博士在PCR和FISH检测及其结果的分析上给予的帮助!感谢首都医科大学病理学系宫丽平教授在FISH检测以及图片的采集方面给予的帮助

摘　　要：目的探讨PCR和FISH检测技术在原发性子宫颈淋巴瘤与淋巴瘤样病变的诊断与鉴别诊断中的作用。方法收集3例原发性子宫颈弥漫性大B细胞淋巴瘤（DLBCL）与2例子宫颈淋巴瘤样病变，进行PCR-IgH重排及FISH检测。结果PCR检测显示3例DLBCL和1例淋巴瘤样病变中出现单克隆性IgH基因重排。间期FISH检测显示3例DLBCL均发生了IgH和bcl-6基因断裂，而2例淋巴瘤样病变均未检测到特定的染色体断裂。结论PCR—IgH重排并非仅见于子宫颈细胞淋巴瘤。间期FISH检测IgH和bcl-6基因的断裂对于子宫颈B细胞淋巴瘤和淋巴瘤样病变的诊断和鉴别诊断有帮助。Objective To investigate the role of polymerase chain reaction （PCR） and fluorescence in situ hybridization （FISH） in diagnosis and differential diagnosis of primary lymphoma and lymphoma-like lesion of the uterine cervix. Methods Three cases of primary cervical diffuse large B-cell lymphoma （DLBCL） and two cases of cervical lymphoma-like lesion were retrospectively studied by detecting their PCR- IgH rearrangement and chromosomal break of IgH, bcl-6, bcl-2 and myc genes with FISH. Results PCR- IgH gene rearrangement analysis demonstrated that the monoclonal rearrangement of IgH gene was occurred in 3 cases of cervical lymphoma and 1 case of lymphoma-like lesion. Interphase FISH analysis detected IgH gene break and bcl-6 gene break in all the three cases of cervical lymphoma, while no specific chromosomal break of IgH, and bcl-6, bcl-2 and myc genes was detected in two cases of cervical lymphoma-like lesion. Conclusion PCR-IgH gene rearrangement is not specific to cervical B-cell lymphoma. Analysis of IgH gene break and bcl-6 gene break with interphase FISH is helpful to differential diagnosis between B-celt lymphoma and lymphoma-like lesion of the uterine cervix.

关 键 词：淋巴瘤  非霍奇金 淋巴瘤  大细胞  弥漫型 淋巴瘤样病变 免疫组织化学 聚 合酶链反应 原位杂交 荧光 

分 类 号：R737.33[医药卫生—肿瘤] R711.74[医药卫生—临床医学]