假单胞菌DN2对多氯联苯的降解及bphA1核心序列测定  

Degradation of Polychlorinated Biphenyls and Confirm of bphA1 Gene Core by Pseudomonas DN2

作　　者：任何军[1] 刘娜[1] 高松[1] 张兰英[1] 张玉玲[1] 周睿[1] 

机构地区：[1]吉林大学环境与资源学院/吉林省水资源与水环境重点实验室,长春130026

出　　处：《吉林大学学报（地球科学版）》2009年第2期312-316,共5页Journal of Jilin University：Earth Science Edition

基　　金：国家自然科学基金项目(50879029)

摘　　要：从长期受PCBs污染的土壤中经富集培养,筛选分离到1株能以联苯为唯一碳源和能源生长的革兰氏阴性细菌DN2。16SrDNA序列分析初步鉴定为Pseudomonassp,利用变压器油测定了该菌对各不同氯取代同系物的降解率。结果表明,DN2可以高效降解多氯联苯,其对10 mg/L的Aroclor 1242总量降解可达67%左右。进一步对该菌的bphA1基因核心序列克隆和测序分析表明,与Pseudomonassp kks102菌株的同源性最高为92%,核苷酸序列转换成氨基酸序列(202AA)后,经ClauxW2比对发现11个保守位点,具有Glu-X3-4-Asp-X2-His-X4-5-His结构域存在。A polychlorinated biphenyls-degrading DN2, which can use biphenyl as the sole carbon source and energy, was isolated from the long-term PCBs-contaminated soil by enrichment in the soil first. According to 16S rDNA sequence analysis results,the strain was identified as Pseudomonas sp preliminarily. This pure strain was tested for biodegradation of several different PCBs by grew cells with transformer oil as food. The results showed the isolated strain degraded up to 67% of 10 mg/L Aroclor 1242. The bphA1 gene core, encoding the catalytic site of the large subunit of biphenyl dioxygenase, was also detected by PCR amplification and confirmed by DNA sequencing. It＇s also showed Glu- X3 - 4 - Asp- X2 - His- X4 - 5 - His domain existed in its constitution by bphA1 gene core.

关 键 词：PCBS 生物降解 bphA1 假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]