荧光分光光度法测定痕量铜——基于铜(Ⅱ)水杨基荧光酮-乳化剂OP-β-环糊精荧光熄灭反应体系  

Fluorospectrophotometric Determination of Trace Amount of Copper Based on the Fluorescence Quenching Reaction System of Cu(Ⅱ)-SAF-OP-β-CD

作　　者：陈世界[1] 李英杰[1] 殷广明[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《理化检验（化学分册）》2009年第3期324-325,329,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：在pH 6.4的磷酸盐介质中,有铜(Ⅱ)存在时,由反应体系水杨基荧光酮(SAF),β-环糊精(-βCD)及乳化剂OP所产生的荧光,由于铜(Ⅱ)与SAF反应生成了配合物而产生猝灭现象,而且荧光强度的减弱程度与铜(Ⅱ)的质量浓度在0.24 mg.L-1以内呈线性关系。荧光检测系在激发波长(eλx)365 nm和发射波长(eλm)523 nm条件下进行。基于上述事实,提出了测定痕量铜的荧光分光光度法,在应用此方法测定自来水中痕量铜时,测得方法的平均回收率为100.6%,平均相对标准偏差(n=6)为0.24%。It was found that the fluorescence emitted by the reaction system of salicylfluorone （SAF）,β-CD and OP, in a phosphate buffer medium of pH 6.4, was quenched by the presence of cupric ion due to the formation of a coordination complex between Cu（ Ⅱ） and SAF. Magnitude of decrease in fluorescence intensity （△F） was found to keep linear relationship with concentration of cupric ion in the range within 0. 24 mg ·L-1 of solution, when the fluorescence measurement was made at the wavelengths of （λex） 365 nm and （λem） 523 nm. Based on these facts, a fluorescence quenching fluorospectrophotometric method for determination of trace amounts of copper was proposed, and in its application of determination of trace amounts of copper in tape water, values of average recovery and average RSD＇s （n=6） found were 100. 6% and 0.24% respectively.

关 键 词：荧光分光光度法 荧光猝灭 铜(Ⅱ) 水杨基荧光酮 乳化剂OP Β-环糊精 

分 类 号：O657.31[理学—分析化学]