唑来膦酸对肺癌95D细胞增殖和侵袭抑制作用的研究  

Inhibition of Zoledronic Acid on Cell Proliferation and Invasion of Lung Cancer Cell Line 95D

作　　者：李明明[1] 张卫东[1] 宗志红[2] 胡振华[1] 王旭晖[1] 

机构地区：[1]天津市第一中心医院胸外科,天津300192 [2]中国医科大学基础医学院生物化学教研室,沈阳110001

出　　处：《中国肺癌杂志》2009年第3期198-202,共5页Chinese Journal of Lung Cancer

摘　　要：背景与目的异常增殖和转移是恶性肿瘤的基本特征,本研究旨在探讨唑来膦酸(zoledrnic acid, ZOL)对肺癌95D细胞增殖和侵袭能力的抑制作用。方法采用MTT法药物敏感性试验,Western blot、RT-PCR和免疫荧光检测细胞增殖、转移能力相关蛋白和mRNA水平的变化,Transwell侵袭小室测定细胞体外侵袭力。结果ZOL对肺癌95D细胞的抑制作用呈时间、剂量依赖性,随着ZOL作用时间的延长,VEGF、MMP9、MMP2的mRNA表达和VEGF、MMP9、ERK1/ERK2的蛋白表达量均逐渐减少。Transwell侵袭实验结果表明,ZOL作用4 d、6 d组侵袭细胞数较对照组明显减少。结论ZOL对肺癌95D细胞的细胞增殖有抑制作用,并能减弱其侵袭能力。Background and objective Abnormal proliferation and metastasis is the basic characteristic of malignant tumors. The aim of this work is to explore the effects of zoledronic acid on cell proliferation and invasion in lung cancer cell line 95D. Methods The effect of zoledmic acid （ZOL） on proliferation of lung cancer cell line 95D was detected by MTT. The expression of proliferation and invasion-relation genes and proteins were detected by Western blot, RT-PCR and immunofluorescence. Changes of invasion of lung cancer cell numbers were measured by polycarbonates coated with Matrigel. Results ZOL could inhibit the proliferation of lung cancer cell line 95D in vitro in a time-dependant and a dose-dependant manner. With time extending after ZOL treated, the mRNA expresion of VEGF, MMPg, MMP2 and protein expression of VEGF, MMP9, ERK1/ERK2 were decreased. The results of Tanswell invasion showed the numbers of invasive cells were significantly reduced in 95D cells treated with ZOL 4 d and 6 d later. Conclusion ZOL could inhibit cell proliferation and invasion of lung cancer cell line 95D.

关 键 词：唑来膦酸 肺肿瘤 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]