人类肺癌组织中COX-2的表达及其与K-ras和Mcl-1表达的关系  

The Expression of COX-2 in Human Lung Cancer and its Relationship with Expression of K-ras and Mcl-1

作　　者：宋文静[1] 王新允[1] 郑宏伟[1] 

机构地区：[1]天津医科大学病理学教研室,天津300070

出　　处：《中国肺癌杂志》2009年第3期216-221,共6页Chinese Journal of Lung Cancer

摘　　要：背景与目的环氧化酶-2(Cyclooxygenase-2,COX-2)是花生四烯酸代谢限速酶的一个亚型,已发现COX-2与肿瘤的发生、发展和转移有关。本文旨在探讨肺癌组织中COX-2表达的临床病理学意义,及其与K-ras和Mcl-1表达的相互关系。方法应用免疫组织化学染色及图像分析系统检测COX-2、K-ras和Mcl-1在101例肺癌及7例正常肺组织中的表达。结果COX-2在非小细胞肺癌组织中高表达(P<0.05),以腺癌表达最高;在周围型肺癌(P<0.05)及中晚期肺癌组织中高表达(P<0.05);并且COX-2的表达与K-ras和Mcl-1的表达呈正相关。结论COX-2的表达与K-ras的基因突变和Mcl-1的表达上调有关,在肺癌的分化、进展过程中具有重要作用。Background and objective Cyclooxygenase-2 （COX-2） is a subtype of rate-limiting enzyme in arachidonic acid metabolism, and it is related with tumorignesis, development and metastasis. In this study, we investigated the expression and clinical significance of COX-2 and its relationship with expression of K-ras and Mcl-1 proteins. Methods The expression of COX-2, K-ras and Mcl-1 were detected in 101 lung cancer tissues and 7 normal lung tissues by immunohistochemical method and image analysis system. Results The overexpression of COX-2 protein was detected in non-small cell lung cancer （P〈0.05）, especially in adenocareinoma; There was overexpression of COX-2 in the peripheral type and late stage lung cancer （P〈0.05）; There was positive correlation among the COX-2, K-ras and Mcl-l（P〈0.05）. Conclusion The expression of COX-2 was closely related with K-ras gene mutation and Mcl-2 upregulation, it played an important role during differentiation and progression of lung cancer.

关 键 词：肺肿瘤 环氧化酶-2 K-ras癌基因 髓细胞白血病因子-1 免疫组织化学 图像分析 

分 类 号：R734.2[医药卫生—肿瘤]