靶向S100A4的shRNA对乳腺癌MCF-7细胞体外侵袭力及迁移力的影响  

Effects of ShRNA-targeted S100A4 on invasion and migration potential of MCF-7 cells in patients with breast cancer

作　　者：马雷[1] 吴爱国 纪术峰[1] 杨华峰[1] 

机构地区：[1]南方医科大学附属珠江医院,广东广州510282

出　　处：《山东医药》2009年第3期22-25,共4页Shandong Medical Journal

基　　金：广东省科技计划资助项目(2008B030301345)

摘　　要：目的探讨靶向S100A4短发夹RNA(shRNA)对MCF-7细胞侵袭力和迁移力影响的作用机制。方法构建S100A4基因特异性shRNA表达载体,使用QRT-PCR和Western blot检测转染后MCF-7细胞中S100A4的表达水平,经过脂质体介导将S100A4-shRNA表达载体转染入MCF-7细胞,G418筛选及克隆化培养。采用Trans-well小室法和划痕试验检测MCF-7细胞侵袭力和迁移力变化。结果经测序鉴定证实S100A4-shRNA表达载体成功构建。转染48h后,所构建的S100A4-shRNA-1表达载体能有效抑制MCF-7细胞中S100A4表达;MCF-7细胞形态无显著变化,但侵袭力和迁移力明显下降。结论S100A4在乳腺癌细胞侵袭和转移过程中扮演重要角色,通过沉默其表达可抑制MCF-7的侵袭力和迁移力从而抑制其转移。Objective To study the effects of short hairp in RNA（shRNA） targeting S100A4 on the invasion and migration potential of breast cancer MCF-7 cells. Methods The S100A4-shRNA expression vector was constructed and conformed by sequencing. S100A4-shRNA expression vector was transfected into MCF-Tcells via lipofectamine TM 2000, and the changes of S100A4 were determined by using QRT-PCR and Western blot after transfection. S100A4-shRNA expression vector was transfected into breast cancer MCF-7 cells by lipofectamine TM 2000 , followed by G418 selection, colon culture. Invasion and migration capability of stably transfected MCF-7 cells in vitro was evaluated by using Transwell chamber model and wound assay. Results S100A4-shRNA expression vector was constructed and transfected into MCF-7cells. 48 h after transfection, S100A4 mRNA and S100A4 protein decreased significantly. The morphous of stably transfected MCF- 7 cells didn＇ t change notably, but the invasion and migration potentiality in whih decreased significantly. Conclusion S100A4 play an important role in the invasion and metastasis of breast cancer. The possible mechanism may inhibit metastasis of MCF-7 cells by decreasing its invasion and migration capability.

关 键 词：乳腺癌 S100A4 SHRNA 侵袭力 迁移力 

分 类 号：R737.9[医药卫生—肿瘤]