人参皂苷Rb1对内源性Aβ所致小鼠神经细胞Tau蛋白过磷酸化的干预作用  

Interventional effect of Ginsenosides Rb1 on Tau protein phosphorylation of neuronal cells induced by endogenous Aβpeptide

作　　者：李国栋[1] 袁保梅[1] 鄢文海[2] 邢莹[2] 

机构地区：[1]郑州大学,河南郑州450052 [2]郑州大学医学院

出　　处：《山东医药》2009年第3期26-28,共3页Shandong Medical Journal

基　　金：河南省自然科学基金项目(0611043000)

摘　　要：目的探讨人参皂苷Rb1对神经干细胞的保护作用及机制。方法将人参皂苷Rb1作用于APP转基因鼠分化中的神经细胞,用免疫荧光化学和免疫蛋白印记法测定总Tau蛋白磷酸化水平及Tau蛋白在Ser-396、Ser-262位点磷酸化水平。结果与阳性对照组相比,人参皂苷Rb1处理组Tau蛋白的总磷酸化水平及在Ser-396和Ser-262位点磷酸化水平明显下降;而与阴性对照组相比,Tau蛋白总磷酸化水平及在Ser-396和Ser-262位点的磷酸化水平明显增高。结论人参皂苷Rb1具有一定神经保护作用;其机制可能为直接促进神经细胞活性或通过调节Tau蛋白上游作用因子的活性。Objective To explore the protective effect and the possible mechanism of Ginsenosides Rbl on the neural stem cells. Methods Western blotting and inmmunol fluorence staining were used to detect the level of Tau protein phosphorylation with the total of Tau-5, the site of Tau Ser-396 and Ser-262 phosphorylation. Results After exposure to Ginsenosides Rbl ,the level of Tau protein and Tau protein phosphorylation in the sites of Ser-396, Ser-262 decreased compared with the APP positive neural cell, compared with to the APP negative neural cell. Conclusion Ginsenosides Rbl can protect nerve function, the possible mechanisin may promote the activity of neural cells or regulate phosphorylation level of Tau protein.

关 键 词：APP转基因小鼠 TAU蛋白 过磷酸化 人参皂苷RB1 

分 类 号：R741.02[医药卫生—神经病学与精神病学]