硫酸铜对玉米根尖细胞遗传毒性效应的研究  

Cytogenetic Toxic Effects of Copper Sulfate on Root Tip Cells of Zea mays L

作　　者：易岚[1] 李国庆[1] 赵欣[1] 贺庆芝[1] 杨露青[1] 刘运莲[1] 秦志峰[1] 

机构地区：[1]南华大学生命科学与技术学院生物科学系,湖南衡阳421001

出　　处：《环境与健康杂志》2009年第3期220-221,共2页Journal of Environment and Health

摘　　要：目的研究硫酸铜对玉米根尖细胞遗传毒性效应。方法以玉米根尖为实验材料,用0.05、0.10、0.25、0.50、1.00g/L的硫酸铜溶液为诱变剂,处理24h,测定玉米根尖细胞的有丝分裂指数、微核率和染色体畸变率。结果与对照组比较,0.05、0.10、0.25、0.50、1.00g/L硫酸铜溶液组玉米根尖细胞的微核率和有丝分裂指数以及0.05、0.10、0.25、0.50g/L硫酸铜溶液组玉米根尖细胞的染色体畸变率较高,差异均有统计学意义(P<0.05,P<0.01)。结论硫酸铜对玉米根尖细胞有明显的遗传毒性效应。Objective To investigate the cytogenetic toxic effects of copper sulfate on root tip cells of Zea mays L Methods The root tip cells of Zea mays L were treated by copper sulfate as mutagen at the doses of 0.05,0.10,0.25,0.50,1.00 g/L for 24 hours. The mitotic index, the micronucleus rate and the chromosome aberration rate of tip cells of Zea mays L were measured. Results Compared with the negative control group, the micronucleus rates and the mitotic index in 0.05,0.10,0.25,0.50,1.00 g/L groups and the chromosome aberration rates in 0.05,0.10,0.25,0.50 g/L groups increased significantly （P〈0.05,P〈0.01）. Conclusion Copper sulfate has cytogenetic toxic effects on the root tip cells of Zea mays L.

关 键 词：硫酸铜 玉米根尖细胞 微核 染色体畸变 有丝分裂指数 

分 类 号：R994.6[医药卫生—毒理学]