大黄素对人肝癌HepG2细胞AIF和EndoG蛋白表达的影响  

Effects of Emodin on Expressions of AIF and EndoG in Human Hepatoma Cell Lines HepG2

作　　者：李杰玉[1] 陈泽奇[1] 林源[1] 李密[1] 黄娟[1] 熊丽丽[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《现代生物医学进展》2009年第5期829-831,共3页Progress in Modern Biomedicine

基　　金：湖南省自然基金项目(No06JJ20050);湖南省中医药科研计划项目(No2008017)

摘　　要：目的:观察大黄素干预后,体外培养的人肝癌HepG2细胞株胞浆和胞核内AIF和EndoG的表达情况,探讨大黄素对非Cas-pase依赖细胞凋亡的影响。方法:将体外培养的HepG2细胞分为四组:空白对照组、Caspase抑制剂组、大黄素干预组、大黄素+Caspase抑制剂组;取对数生长期的细胞进行实验,药物作用48小时后提取各组细胞胞浆及胞核蛋白;采用Western Blotting法分别检测各组细胞胞浆和胞核内AIF与EndoG的表达,并进行统计学分析。结果:AIF和EndoG在大黄素干预组、大黄素+Cas-pase抑制剂组细胞胞浆和胞核的表达均高于空白对照组及Caspase抑制剂组(p<0.01),其中以大黄素+Caspase抑制剂组的表达最高(p<0.01)。结论:大黄素能促进体外培养的人肝癌HepG2细胞AIF和EndoG的表达和核转位,可以通过非Caspase依赖途径诱导细胞凋亡。Objective： To study the effects of emodin on the expression and nuclear translocation of AIF and EndoG in human hepatoma cell HepG2 and explore its mechanism in inducing apoptosis. Methods： The human hepatoma cells HepG2 cultured in vitro were divided into 4 groups： blank control group, Caspase inhibitor group, Emodin group and Emodin combined with Caspase inhibitor group. The expression of AIF and EndoG in endochylema and nucleus were detected by Western Blotting, respectively. Results： The pro- tein expression of AIF and EndoG in Emodin group and Emodin combined with Caspase inhibitor group were higher than that in blank control group and Caspase inhibitor group （P〈0.01）, and that in Emodin combined with Caspase inhibitor group was the highest expres- sion. Conclusions： Emodin can up-regulate the protein expression and translocation of AIF and EndoG in human hepatoma cell HepG2, Emodin can induce apoptosis through Caspase-independent pathway.

关 键 词：大黄素 肝细胞癌 凋亡诱导因子 核酸内切酶G 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]