水稻果皮色泽近等基因系的构建及近等性评价  

Construction of Near Isogenic Lines for Pericarp Color and Evaluation on Their Near Isogenicity in Rice

作　　者：王晓光[1] 季芝娟[1] 蔡晶[1] 马良勇[1] 李西明[1] 杨长登[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006

出　　处：《中国水稻科学》2009年第2期135-140,共6页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2006AA10Z1B5);浙江省重大科技专项重点资助项目(2006C12091);浙江省水稻育种协作攻关专项资助项目(2007C12904);农业部超级稻专项资助项目(200606)

摘　　要：以紫香糯和春江糯2号为亲本,利用F2、BC1F1和F3分离群体对果皮色泽性状进行遗传分析,并构建了7对果皮色泽近等基因系。遗传分析结果表明紫香糯果皮的色泽性状由两对互补基因控制。对7对近等基因系进行的性状相似性和遗传背景的SSR分子标记检测结果表明,近等基因系间在农艺性状上除了千粒重外,其他性状差异均不显著。利用34对亲本间多态性SSR分子标记,发现5对黑、白近等基因系仅在目标基因区段检测到了多态性标记,近等性比较理想;2对黑、褐近等基因系除了目标区段外,还分别在第11、12染色体检测到了多态性标记,近等性较差。The inheritance of purple pericarp was studied through genetic analysis using F2, BC1 F1 and F3 from a cross between a purple pericarp rice variety Zixiangnuo and a white pericarp rice variety Chunjiangnuo 2. Seven pairs of near isogenic lines（NILs） for pericarp color of rice were further constructed. The genetic analysis indicated that the purple pericarp was controlled by two complementary genes （Pb and Pp）. Analyses on agronomic traits showed that no significant differences on agronomic traits were found among NILs except grain weight. The polymorphic SSR markers for the two parents were detected only in target segments of the five pairs of NILs different in Pb, which revealed that the near isogenicities of them were fine. However, for the two pairs of NILs different in Pp, the polymorphic markers for the two parents were detected as well as in non-target segments of chromosomes 11 and 12, respectively.

关 键 词：果皮色泽 近等基因系 农艺性状 分子标记 近等性 水稻 

分 类 号：S511[农业科学—作物学]