探索学习对局灶性脑梗死大鼠梗死灶周围皮质Ng及bFGF表达的影响  

The effects of learning on Ng and bFGF expressions in peri-infarction cortex of rats after unilateral local cerebral infarction

作　　者：槐雅萍 贾新凤[1] 闫桂芳[1] 贾子善[1] 

机构地区：[1]河北省人民医院康复中心,石家庄050051

出　　处：《中国康复医学杂志》2009年第3期216-218,共3页Chinese Journal of Rehabilitation Medicine

基　　金：河北省科技厅资助课题(072761D-137)

摘　　要：目的:观察探索学习对局灶性脑梗死大鼠神经颗粒素(Ng)及碱性成纤维细胞生长因子(bFGF)表达的影响。方法:健康雄性SD大鼠70只,其中60只电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索学习组(n=30)和对照组(n=30),假手术组(n=10)仅开颅不电凝大脑中动脉,居于标准笼。探索学习组和对照组分别于术后第1天、7天、14天、28天各组随机选取5只大鼠处死,假手术组分别于7天、28天时随机选取5只大鼠处死,即进行Ng及bFGF免疫组化染色,测定其在脑梗死灶周围皮质的表达情况。结果:探索学习组Ng及bFGF阳性神经元数在第7天、14天、28天明显多于对照组(P<0.01)。结论:探索学习能促进梗死灶周围皮质Ng及bFGF表达。Objective： To study the effects of learning on neurogranin （Ng） and basic fibroblast growth factor（bFGF） expressions in peri-infarction cortex of） rats after unilateral local eerebral infarction. Method：Seventy male Sprague- Dawley rats were adopted. After the models of MCAO were established by electric coagulation successfully, rats were randomly divided into learning group （n=30, living in maze cages）, control group （n=30, every 5 rats as a subgroup living in large standard cages） and Other 10 rats didn＇t receive electric coagulation at middle cerebral artery, as sham group living in standard cage. At the 1^th, 7^th, 14^th, 28^th d after MCAO, in learning group and control group 5 rats were randomly sacrificed separately. In sham group 5 rats were randomly sacrificed separately at the 7^th, 28^th d after operation. The expressions of bFGF and Ng in peri-infarction cortex were examined by immunohistochemistry staining. Result： The number of Ng and bFGF labeled ceils in learning group was significomtly more than those in control group at 7^th, 14^th and 28^th day（P〈0.01）. Conclusion： Learning could promote expressions of Ng and bFGF in peri-infarction cortex of rats after unilateral local cerebral infarction.

关 键 词：探索学习 脑梗死 大鼠 神经颗粒素 碱性成纤维细胞生长因子 

分 类 号：R743.33[医药卫生—神经病学与精神病学]