从废弃蛹虫草大米培养基中高效提取纯化虫草素工艺条件研究  

An efficient method of extracting and purifying cordycepin from waste rice medium of Cordyceps militaris

作　　者：韦会平[1,2] 叶小利[3] 张华英[2] 李学刚[1] 钟运俊[3] 

机构地区：[1]西南大学药学院,重庆400716 [2]攀枝花学院医学院,攀枝花617000 [3]西南大学生命科学院,重庆400716

出　　处：《菌物学报》2009年第2期220-225,共6页Mycosystema

基　　金：国家自然科学基金(No.20673084);重庆市卫生局医学科技项目(渝中医[2001]25)

摘　　要：虫草素是蛹虫草的次生代谢产物,具有重要的药用价值,当前主要从蛹虫草子实体中提取。本文提供了一种从蛹虫草子实体生产的副产物——大米培养基中高效提取纯化虫草素的新方法。该方法包括连续逆流提取、732阳离子交换树脂柱层析和结晶三步。对每一步的工艺参数进行了系统优化。用该优化的方法从大米培养基中提取虫草素,虫草素产品的纯度达98.0%以上,产率达66.0%以上。Cordycepin is an important secondary metabolite of Cordyceps militaris, a traditional Chinese medicinal fungus, and is mainly obtained from fruiting bodies at present. This article presents an efficient method of extracting and purifying cordycepin from rice medium, the waste of fruiting body production. The method included 3 steps： continuous countercurrent extraction, column chromatography with 732 cation exchange resin, and crystallization, and their working parameters were optimized. By use of the optimized method, cordycepin of purity over 98.0% was obtained, with the yield over 66.0%.

关 键 词：药用真菌 生产废物利用 连续逆流提取 柱层析 结晶 

分 类 号：S567.35[农业科学—中草药栽培] TS264.4[农业科学—作物学]