WCX纳米磁珠在宫颈鳞癌血清蛋白质组中的应用及其临床意义  

Application of WCX magnetic bead for serum proteome profiling in cervical squamous cell carcinomas and its clinical significance

作　　者：郑智国[1] 夏婷[2] 李永哲[3] 高赟[1] 牟瀚舟[1] 许沈华[1] 许洋[4] 

机构地区：[1]浙江省肿瘤研究所 [2]浙江省肿瘤医院妇瘤科,浙江杭州310022 [3]中国医学科学院北京协和医学院北京协和医院,北京100730 [4]中国医学科学院北京协和医学院检验医学研发中心临床蛋白质组学研究室,北京102206

出　　处：《基础医学与临床》2009年第3期287-291,共5页Basic and Clinical Medicine

摘　　要：目的研究宫颈鳞癌患者血清蛋白,筛选差异蛋白并建立诊断模型,探讨其临床意义。方法用WCX纳米磁珠联合基质辅助激光解析离子化飞行时间质谱检测77例宫颈鳞癌,13例宫颈上皮内瘤变Ⅲ级患者和52名健康人的血清。用Biomarker Wizard软件分析并找出差异蛋白,再用Biomarker Patterns软件建立诊断模型。同时与SCC-Ag作比较。结果建立了由3974、3398和13732等差异蛋白峰组成的宫颈癌诊断模型,其敏感性为100%(32/32),特异性为93.8%(30/32)。扩大样本验证,其敏感性为77.8%(35/45),特异性为75%(15/20)。另外13例CINⅢ级患者有11例被检出,34例SCC-Ag阴性的患者中有30例被检出。结论由3974、3398和13732三个差异蛋白组成的诊断模型有助于区分宫颈鳞癌,CINⅢ级患者与健康人。同时也能检出SCC-Ag阴性的宫颈鳞癌患者。Objective To analyze the serum proteomic pattern of the cervical cancer patients, to develope diagnostic model and to evaluate its clinical significance. Methods WCX magnetic bead and MALDI-TOF were used to detect the serum proteomic pattern of 77 patients with cervical squanmous cell carcinomas, 13 patients with CIN Ⅲ and 52 healthy women. Biomarker Wizard software was used to detect protein peaks and potential difference between cervical cancer and controls. The model was developed by Biomarker Patterns software. Results A diagnostic pattern consisting of three differential protein peaks was established with 100% （32/32）sensitivity and 93.8% （ 30/32 ） specificity. A sensitivity of 77.8 % （ 35/45 ） and a specificity of 75 % （15/20） in blind test were obtained. The diagnostic model also could discriminate CIN Ⅲ and SCC-Ag negative patients from controls. Conclusion The diagnostic pattern combining 3974,3398,13732m/z protein peaks can discriminate not only cervical squamous cell cancer but also CIN m and SCC-Ag negative patients from controls.

关 键 词：宫颈癌 基质辅助激光解析离子化飞行时间质谱 纳米磁珠 

分 类 号：R737.33[医药卫生—肿瘤]