小肠腺癌中错配修复基因hMLH1的表达及意义  

Expression of DNA Mismatch Repair Gene hMLH1 and Its Significance in Small Bowel Adenocarcinoma

作　　者：李小燕[1] 黄缘[1] 

机构地区：[1]南昌大学第二附属医院,330006

出　　处：《肿瘤防治研究》2009年第3期207-209,共3页Cancer Research on Prevention and Treatment

摘　　要：目的探讨DNA错配修复基因hMLH1在小肠腺癌中的表达及临床意义。方法应用免疫组化SP法检测36例小肠腺癌及癌旁正常组织中hMLH1基因蛋白表达情况。结果hMLH1蛋白表达率在小肠腺癌组(66.7%)低于癌旁正常组(91.7%),两组间差异有统计学意义(P<0.05)。hMLH1失表达与患者年龄、性别、肿瘤部位、大小、浸润深度、有无淋巴结转移无关(P>0.05),但是与肿瘤分化程度密切相关(P<0.05),分化程度越低,hMLH1失表达越明显。结论小肠腺癌中存在MMR基因的缺陷,hMLH1基因可作为判断肿瘤分化程度的一个生物学指标。Objective To investigate the expression of DNA mismatch repair gene hMLH1 and its clinical significances in small bowel adenocarcinoma（SBA）. Methods hMLH1 expression was detected on cancerous and adjacent normal tissues from 36 cases of SBA using IHC （SP method）. Results Positive rate of hMLH1 protein was lower in SBA （66. 7%） than in tumor-adjacent tissuses （91.7%）. Significant difference was found between the expression of cancerous and adjacent normal tissues. The hMLH1 protein expression was significantly correlated with the degree of tumor cell differentiation （P〈0. 05）, but no correlation with other clinicopathologic parameters such as age, sex, tumor size, location, invasive depth and lymph node metastasis （P〈0. 05 ）. Conclusion There was a subset of SBA accompanying MMR defect, hMLH1 gene can be used as a target to evaluate the tumor differentiation.

关 键 词：HMLH1 错配修复基因 小肠肿瘤 免疫组织化学 

分 类 号：R735.32[医药卫生—肿瘤]