食管鳞状细胞癌中Wnt2、β-catenin、c-myc和cyclinD1的表达及临床意义  

Expression and Significance of Relevant Factors of Wnt2,β-catenin,c-myc and cyclin D1 in Esophageal Squamous Cell Carcinoma Tissue

作　　者：王伟[1] 刘红涛[2] 柴玉荣[3] 许培荣[2] 王鹏举[3] 刘兰琦[2] 薛乐勋[2] 

机构地区：[1]郑州大学第一附属医院细胞生物教研室,450052 [2]郑州大学生物系 [3]郑州大学基础医学院

出　　处：《肿瘤防治研究》2009年第3期213-215,220,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究Wnt2、β-catenin及其下游的靶基因c-myc、cyclin D1在食管鳞癌中的表达情况,以期探讨其在食管鳞癌发生发展中的作用及意义。方法用免疫组织化学SP法检测40例食管鳞癌患者石蜡包埋的癌组织、癌旁组织及正常组织中Wnt2、β-catenin、c-myc、cyclin D1表达情况,分析Wnt2、β-cate-nin和c-myc、cyclin D1表达之间的相关性及与临床病理特征的关系。结果Wnt2、β-catenin和c-myc、cyclin D1在食管癌组织、癌旁组织及食管正常组织中表达阳性率逐渐降低,差异有统计学意义(P<0.01),β-catenin和c-myc与肿瘤浸润和有无淋巴结转移相关(P<0.05)。结论Wnt2/β-catenin/Tcf4通路在食管鳞状细胞癌发生发展中起重要作用,该通路有可能成为基因治疗的潜在靶点。Objective To investigate the expression of Wnt2,β-catenin and their target genes such as c-myc,cyclinD1 in tissues of esophageal squamous cell carcinoma （ESCC）. Methods The expression level of Wnt2, β-catenin, c-myc, cyclin D1 in 40 cases of esophageal squamous cell carcinoma was detected by immunohistochemistry, and its correlation with clinicopathological features was analyzed. Results The aberrant expressions of Wnt2, β-catenin, c-myc and cyclinD1 in ESCC was higher than tissue adjacent to cancer and normal tissues, there was a significant difference among the groups（P〈0. 01）. The expressions of β-catenin and c-myc protein were closely correlated with infiltrative depth and lymphatic metastasis in esophageal squamous cell carcinoma ESCC（P〈0. 05）. Conclusion Wnt2/β-catenin /Tcf4 signal pathway plays an important role in ESCC and may become a prognostic factors or gene therapy target for ESCC.

关 键 词：食管肿瘤Wnt2 Β-CATENIN 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]