缺血再灌卒中后抑郁小鼠模型的建立  

Model Establishment of Post-stroke Depression in Mice

作　　者：邢东明[1] 国月英[2] 炎彬[1] 王道毅[1] 王玉刚[1] 杜力军[1] 

机构地区：[1]清华大学生物科学与技术系药物药理研究室,北京100084 [2]中国中医科学院西苑医院,北京100083

出　　处：《中国比较医学杂志》2009年第3期14-19,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(30572340,90713043);教育部博士学科点基金(20060003072,20070003039)资助

摘　　要：目的探讨并建立卒中后抑郁小鼠动物模型。方法先利用结扎颈总动脉造成小鼠不完性全脑缺血再灌注病理损伤,利用悬尾和强迫游泳方法诱导小鼠抑郁模型,利用正交试验设计全面考察其影响因素,确定最佳造模条件。结果以缺血5 min,再灌10 min,再缺血5 min所诱导抑郁模型最为明显,且死亡率较低。结论利用此方法所造卒中后抑郁模型具有操作简便、成功率较高且经济的特点,适用于该类药物筛选。Objective To study the model of post-stroke depression （PSD） with cerebral ischemia-reperfusion in mice. Methods First, the pathological changes of cerebral ischemia- reperfusion by ligaturing the cephalic arteries, then depression like behavior （immobility time） induced by tail suspension and forced swimming; the alteration analysis carried out by crossing test design. Results The best process of the model-induee is 5 minutes of blood isehemia, 10 minutes of blood reperfusion and 5 minutes of blood ischemia. Conclusion The model by the assay will be used for candidate drug screening in post stroke depression with simple operating, lower mortality and in economy.

关 键 词：卒中后抑郁 小鼠 模型 

分 类 号：R743[医药卫生—神经病学与精神病学]