一种新型DHA复合物的抑瘤作用及对Caspase-3凋亡蛋白表达的影响  

The Anti-tumor Effect of New Kind of DHA Compound and Its Effect on Caspase-3 Expression

作　　者：刘艳[1] 姚文环[2] 孙克任[3] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]山东省疾病预防控制中心,济南250014 [3]山东大学公共卫生学院,济南250012

出　　处：《中国比较医学杂志》2009年第3期25-27,49,I0004,共5页Chinese Journal of Comparative Medicine

基　　金：山东省科委基金资助项目(编号:981154309)

摘　　要：目的评价一种新型DHA复合物(AT-158)对荷瘤小鼠移植瘤及体外培养肿瘤细胞的抑瘤作用,并对其可能的作用机制做初步探讨。方法建立移植瘤荷瘤小鼠动物模型和体外细胞培养的方法,评价AT-158在体内和体外的抑瘤作用,观察体外培养的Hela细胞在药物作用下的核形态的改变,测定移植瘤中Caspase-3凋亡蛋白表达的变化。结果AT-158在体内和体外均显示显著的抑瘤作用。肿瘤细胞的核形态发生凋亡细胞特征性改变。诱导凋亡执行蛋白Caspase-3表达增加。结论AT-158显示一定的抑瘤效果,具有较高的开发价值。Objective To evaluate the antitumor effect and elementarily discuss the possible mechanism of AT-158, a kind of DHA compound. Methods The in vivo and in vitro effect was assessed. And then, the structural alteration on the nucleus and the expression of apoptosis associated protein, such as Caspase-3 was studied by fluorescence-microscope, Western blot methods. Results AT-158 showed great cytotoxity toward tumor cells in which the typically morphological changes were founded during apoptosis, such as nucleus condensing and splitting. At the same time, the levels of Caspase-3 was increased after AT-158 treatment. Conclusion It was concluded that AT-158 could effectively inhibit the growth of some kind of tumors, indicating a promising anticarcinogen.

关 键 词：DHA复合物 抑瘤作用 细胞凋亡 CASPASE-3 

分 类 号：R73[医药卫生—肿瘤]