山茱萸总苷对大鼠实验性变态反应性脑脊髓炎的初步观察  

Primarily Research of Total Glycosides from Cornus Officinalis on Experimental Allergic Encephalomyelitis in Rats

作　　者：李勇[1] 宋兴伟[1] 代允义[1] 管璇[2] 王小同[1] 张长国[1] 

机构地区：[1]温州医学院第二附属医院神经内科 [2]温州医学院第二附属医院儿童ICU,325000

出　　处：《中国临床神经科学》2009年第2期118-122,共5页Chinese Journal of Clinical Neurosciences

基　　金：浙江省中医药局课题资助项目(2008YA024)

摘　　要：目的:观察山茱萸总苷(TGCO)对大鼠实验性变态反应性脑脊髓炎(EAE)的影响。方法:Wistar大鼠24只随机分为3组:正常对照组、EAE组和TGCO组。采用动物行为学、常规苏木精-伊红染色和LFB髓鞘染色观察大鼠的发病情况与中枢神经系统(CNS)的病理变化。结果:EAE组7/8只大鼠出现典型的EAE行为学改变、CNS炎性细胞浸润和髓鞘脱失。TGCO组有3/8只大鼠出现EAE行为学改变,评分最高为2分,潜伏期延长,CNS内炎性细胞浸润和髓鞘脱失明显减轻。对照组未见动物行为学和CNS病理学改变。结论:TGCO能减轻EAE大鼠的临床症状、延长潜伏期和降低发病率。Aim： To primarily investigate the effect of total glycosides from comus officinalis（TGCO） on experimental allergic encephalomyelitis（EAE） in rats. Methods： Twenty four Wistar rats were randomly divided into three groups： Control group, EAE group, TGCC group. The diet, movement and body weight of Wistar rats were daily examined, CNS tissues were stained with Hematoxylin-eosin and Luxol Fast Blue. Results： Seven eights rats in EAE group demonstrated neurological signs of typical EAE with parenchymal infiltration and perivascular cuffing with mononuclear cells, and appreaed demyelination in the white matter. However, three eighths rats in TGCO group demonstrated neurological signs of typical EAE（the high score was 2, the latency was prolonged）, the infiltration of mononuclear and myelin damages in CNS were reduced. No infiltration of mononuclear and myelin damages in CNS were noted in all rats of control groups. Conclusion： TGCO could lighten clinical symptom, prolong the latency, and suppress the attack of EAE rats.

关 键 词：多发性硬化 脑脊髓炎 山茱萸总苷 实验性变态反应性脑脊髓炎 

分 类 号：R744.52[医药卫生—神经病学与精神病学]