GLUT1和VEGF在肾癌组织中的表达及其临床意义  

Expression of GLUT1 and VEGF in renal cell carcinomas and their clinical significance meanings

作　　者：许乐[1] 林宗明[1] 陈莲[2] 陈弘[3] 刘宇军[1] 鲁继东[1] 张立[1] 王国民[1] 

机构地区：[1]复旦大学附属中山医院泌尿外科,上海200032 [2]复旦大学附属儿科医院病理科,上海201102 [3]无锡市第二人民医院泌尿外科,江苏无锡214002

出　　处：《复旦学报（医学版）》2009年第2期149-154,共6页Fudan University Journal of Medical Sciences

摘　　要：目的观察葡萄糖转运蛋白1(glucose transporter 1,GLUT1)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在肾透明细胞癌中的表达及其临床意义。方法应用免疫组织化学的方法检测60例肾透明细胞癌患者的肿瘤组织、瘤旁肾组织以及远离肿瘤的正常肾组织中GLUT1和VEGF的表达,并结合肾癌患者的临床病理特征进行比较。用Western blot法来进一步验证肿瘤组织和正常组织中GLUT1表达的差异。结果GLUT1在肾癌组织中的表达高于瘤旁肾组织和正常肾组织(P<0.001);在直径≥5cm的肿瘤中的表达强度高于直径<5cm的肿瘤(P=0.033)。与局限期(TNMⅠ+Ⅱ期)肾癌相比,进展期(TNMⅢ+Ⅳ期)肾癌有高表达GLUT1的趋势(P=0.085)。但在不同分级的肾癌组织中,GLUT1表达没有差异。Western blot检测结果也表明肾癌组织中GLUT1的表达明显高于相对应的正常肾组织。VEGF在肾癌组织中的表达高于瘤旁肾组织和正常肾组织(P=0.001);在直径≥5cm的肿瘤中的表达强度要高于直径<5cm的肿瘤(P=0.017)。进展期肾癌VEGF的表达水平明显高于局限期肾癌(P=0.017)。在不同分级的肾癌组织中,VEGF的表达也没有发现差异。GLUT1的表达与VEGF的表达呈正相关(r=0.307,P=0.017)。结论GLUT1和VEGF的表达与肾透明细胞癌的临床病理特征存在一定联系,两者可能共同参与了肾透明细胞癌的发生、发展。Objective To investigate the protein expression of glucose transporter 1 （GLUT1） and vascular endothelial growth factor （VEGF） in clear cell renal cell carcinomas （RCC） and concomitant renal tissues. Methods Tumor tissues and corresponding peritumoral and macroscopically normal kidney tissues were collected from 60 patients with RCC. The expression of GLUT1 and VEGF was evaluated immuohistochemically in relation to clinic opathlogicat characteristics. Western blot was also applied to confirm the differential protein levels of GLUT1 in tumoral and normal renal tissues. Results GLUT1 and VEGF protein expression was significantly higher in tumoral tissues than in peritumoral and normal tissues （P 〈 0. 05, respectively）. Stronger GLUT1 immunostaining was detected in larger tumors compared with that in smaller ones （P = 0. 033） and in advanced tumors compared with that in localized ones （P = 0. 085）. Western blot showed a markedly increased GLUT1 protein content in cancerous samples than in normal ones, which was in accordance with the finding of immunohistochemistry. There were significant differences in VEGF expression in relation to tumor size and TNM stage （P = 0. 017,respectively）. No significant difference was observed in either GLUT1 or VEGF expression between tumors of different nuclear grades. GLUT1 expression correlated positively with VEGF expression（r ： 0. 307, P = 0. 017）. Conclusions There are associations between the expression of GLUT1 and VEGF and the clinic opathlogical factors of clear cell RCC. Elevated levels of GLUT1 and VEGF may play a role in tumor growth and progression cooperatively.

关 键 词：肾细胞癌 葡萄糖转运蛋白1 血管内皮生长因子 免疫组织化学 WESTERN BLOT 

分 类 号：R737.11[医药卫生—肿瘤]