钛表面塑性变形纳米化对MC3T3细胞黏附的影响  

The effects of nanostructured titanium fabricated via surface plastic deformation on MC3T3 cell adhesion

作　　者：陶凤娟[1] 余优成[1] 陈万涛[2] 万鹏波[2] 叶冬霞[2] 于晓平[1] 王庆[1] 

机构地区：[1]复旦大学附属中山医院口腔科,上海200032 [2]上海交通大学医学院附属第九人民医院口腔颌面外科-上海市口腔医学重点实验室,200011

出　　处：《复旦学报（医学版）》2009年第2期206-211,共6页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(30572053);上海市科学技术委员会纳米专项(0652nm018);上海市重点(优势)学科建设项目(Y0203)

摘　　要：目的研究纯钛表面塑性变形纳米化后对小鼠成骨细胞株MC3T3细胞黏附等生物学行为的影响。方法应用塑性变形技术使纯钛表面纳米化,钛板大小10mm×10mm×1mm。未处理纯钛为对照组(n=6),塑性变形纳米化钛板为实验组(n=6)。扫描电镜观察MC3T3细胞在材料表面的黏附形态,荧光显微镜检测细胞黏附的数目,应用激光共聚焦扫描显微镜观察细胞骨架肌动蛋白的特点。结果扫描电镜下观察表明,MC3T3细胞是以多个大小不等的胞突黏附于纳米化钛板表面,细胞表现良好的伸展形态;荧光显微镜显示,细胞在纳米化钛板表面的黏附能力较表面未处理钛板明显提高(P<0.05);激光共聚焦扫描显微镜下观察发现,纳米化钛板表面结构能促进细胞骨架肌动蛋白纤维的充分铺展。结论纯钛表面塑性变形纳米化后,能明显促进MC3T3细胞在其表面的黏附和铺展,改善了纯钛的生物相容性。Objective plastic deformation on To investigate the effects MC3T3 cell adhesion. of nanostructured titanium fabricated via surface Methods Nanostructured titanium surface was prepared using plastic deformation method. The nanostructured titanium was examined as experimental group,while the untreated titanium was used as control group. The effects of nanostructured titanium surface on MC3T3 cell adhesion, shape and cytoskeletal structure were examined using scanning electron microscope （SEM）, fluorescence microscope and laser confocal scanning microscope （LCSM）. Results The morphological changes observed by SEM showed that MC3T3 cells had better shape on the surface of nanostructured titanium. The nanostructured titanium surface promoted MC3T3 cell adhesion （P〈0%0. 05）. The cells of the experimental group showed more extensive spreading than that of the control group. Conclusions The biological behavior of MC3T3 cells on nanostructured titanium.

关 键 词：钛表面 纳米化 塑性变形 成骨细胞黏附 细胞骨架 

分 类 号：R783.1[医药卫生—口腔医学]