一种新的显示蛛网膜下腔与颈部淋巴管交通的研究方法:Microfil^(TM)蛛网膜下腔注射  

Microfil^(TM) injection into the cisterna magna for displaying connection between subarachnoid space and cervical lymphatic vessels:a new method

作　　者：刘海生[1] 王东[1] 齐岩[2] 李妍[3] 孙朝晖[3] 陈业涛[1] 王云鹏[1] 王世杰[1] 张秋航[2] 

机构地区：[1]清华大学玉泉医院神经外科,北京100049 [2]首都医科大学宣武医院耳鼻咽喉-头颈外科,北京100053 [3]清华大学玉泉医院神经中心实验室,北京100049

出　　处：《中国微侵袭神经外科杂志》2009年第3期122-124,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金(编号:30700858);北京市首都医学发展基金(编号:2005-1035)

摘　　要：目的采用一种新的方法在形态学上证实兔和大鼠蛛网膜下腔与颈部淋巴系统相通。方法在刚死亡的兔和大鼠枕大池内注射MicrofilTM,注射2h后在手术显微镜下观察灌注物质在蛛网膜下腔及头颈部淋巴管内的分布。结果MicrofilTM在兔和大鼠枕大池、矢状窦、嗅球、筛板区域聚集分布,穿过筛板,使嗅黏膜淋巴管明显染色而呈黄色,经鼻咽部淋巴管回流到双侧颈浅和颈深淋巴管。两种动物的MicrofilTM染色范围没有明显差别。结论MicrofilTM蛛网膜下腔注射是显示蛛网膜下腔与颈部淋巴系统相通的一种有效且便捷的方法。Objective To reveal morphological route between subarachnoid space and cervical lymphatic vessels by a new method. Methods Microfil^TM was injected into the cisterna magna of the rabbit and rat that were sacrificed just a moment ago, and its distributions in the olfactory bulbs, olfactory tracts and nasal mucosa were observed by operating microscopy 2 hours later after injection. Results Under the operating microscope, Microfil^TM was found in the subarachnoid space and along the olfactory nerves in rabbit and rat. At the nasal mueosa, a lymphatic network was stained in yellow was identified near the olfactory nerves, which crossed the nasopharyngeal region and finally emptied into the superficial and deep cervical lymph nodes. Between rabbit and rat, there is no difference in the distribution of Mierofil^TM. Conclusion Subarachnoid injection of Microfil^TM is a new, quick and convenient method for showing morphological route between subarachnoid space and cervical lymphatic vessels.

关 键 词：淋巴造影术 脑脊髓液 蛛网膜下腔 中枢神经系统 

分 类 号：R-331[医药卫生]