复合维生素对雨生血球藻生长及虾青素积累的影响  

Effect of combined additions of Vitamin on growth and astaxanthin accumulation of Haematococcus pluvialis

作　　者：李立欣[1] 段舜山[2] 战友[1] 

机构地区：[1]黑龙江科技学院资源与环境工程学院,哈尔滨150027 [2]暨南大学水生生物研究中心,广州510632

出　　处：《黑龙江科技学院学报》2009年第1期12-15,共4页Journal of Heilongjiang Institute of Science and Technology

基　　金：国家自然科学基金项目(30770336);珠海市科技计划重点项目(PC20061045);广东省科技计划重点引导项目(2005B33201001)

摘　　要：采用淡水经济微藻雨生血球藻(Haematococcus pluvialis CH-1)为实验藻种,研究了复合添加维生素B1(VB1)、B12(VB12)和维生素H(VH)对雨生血球藻生长及虾青素质量浓度的影响。实验分别复合添加VB1/VB12、VB1/VH、VB12/VH和VB1/VB12/VH,每组各分6个浓度梯度。在4组复合添加实验中,VB1/VB12、VB1/VH、VB12/VH、VB1/VB12/VH的最佳添加质量浓度分别为100/0.5、100/5、0.5/5和1 000/5/50μg/L。4组处理的细胞密度分别比空白提高了22.3%、21.5%、24.3%和20.9%,虾青素质量浓度分别比空白提高了24.1%、24.0%、26.5%和23.8%。此外,在该实验设定的添加浓度范围内,维生素的添加量为各组的最高浓度时,雨生血球藻的细胞密度和虾青素质量浓度均显著低于空白。结果表明,适量复合添加三种维生素中的两种或三种均具有促进雨生血球藻生长及虾青素积累的作用。The effects of combined additions of Vitamin-B1, Vitamin-Bl2 and Vitamin-H on the growth and astaxanthin content of Haematococcus pluvialis were studied by using an economic microalgae Haematococcus pluvialis as experimental material. The experiment of combined adding six grades of concentrations of VB1/VB12, VB1/VH, VB12/VH, VB1/VB12/VH respectively was conducted. The results show that the optimal concentration of adding VB1/VB12 ,VB1/VH, VB12/VH, VB1/VB12/VH was 100/0. 5,100/5,0. 5/5 and 1 000/5/50 μg/L respectively in four test groups. Cells density was enhanced observably with 22. 3%, 21.5%, 24. 3% and 20. 9% higher than the blank, and content of astaxanthin was 24. 1%, 24.0%, 26. 5% and 23.8% higher than the blank respectively. Additionally, when the highest concentration of every group was added, cells density and astaxanthin content of Haematococcus pluvialis were much lower than the blank. The results show that the growth and astaxanthin accumulation of H. pluvialis were improved significantly by properly combined additions of two or three kinds of Vitamin.

关 键 词：雨生血球藻 复合添加 维生素B1、B12、H 虾青素 

分 类 号：Q949.2[生物学—植物学]