碱茅(Puccinellia tenuiflora)Put-R40g3基因的分离及其与逆境的应答  

Isolation and Characterization of Put-R40g3 Gene in Puccinellia tenuiflora Which Expressed in Response to Abiotic Treatments

作　　者：于雪飞[1] 杨传平[1,2] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [2]东北林业大学林木遗传育种黑龙江省重点实验室,哈尔滨150040

出　　处：《分子植物育种》2009年第2期251-256,共6页Molecular Plant Breeding

基　　金：国家973计划(2009CB119102)资助

摘　　要：从碱茅(Puccinellia tenuiflora)cDNA文库中分离得到Put-R40g3基因(登录号:AB465547)。克隆的cDNA全长为588bp,其编码的蛋白质共195个氨基酸,与水稻,小麦和拟南芥的R40g3蛋白的氨基酸序列有较高的同源性。对经逆境处理的碱茅幼苗根、叶中该基因的表达分析结果表明,碱茅中Put-R40g3基因的表达有被ABA、盐和干旱诱导的趋势。Put-R40g3蛋白与绿色荧光蛋白(GFP)融合在酵母细胞表达的蛋白质定位研究结果显示了Put-R40g3蛋白存在于细胞质中。同时Put-R40g3基因在酵母中表达,酵母在盐、干旱及氧化逆境条件下提高了对逆境的适应能力。这些结果暗示了Put-R40g3基因是一个与逆境相关的基因。Put-R40g3 gene （accession number： AB465547） was isolated from Puccinellia tenuiflora. The ORF of Put-R40g3 gent is 588 bp which encoded 195 amino acids and showed high similarity to R40g3 protein in Triticum aestivum, Oryza saiiva, and A rabidopsis thaliana. RT-PCR analyses showed that the expression of Put-R40g3 gene in root and leaf of P. tenuiflora was induced by several abiotic stresses from salts, drought and ABA treatment. The Put-R40g3 protein was localized in cytoplasm by using the green fluorescent protein （GFP） marker. Transgenic yeast expressing Put-R40g3 gone increased the resistance to salt, drought, oxidative stresses and ABA treatment, which indicate Put-R40g3 gene is related to abiotic stresses.

关 键 词：碱茅 ABA 逆境 GFP 酵母 

分 类 号：Q943[生物学—植物学]