水牛伊氏锥虫间接ELISA检测方法的建立  

Establishment of an Indirect ELISA for Trypanosoma evansi in Water Buffaloes

作　　者：何木荣[1] 曾小飞[1] 陈汉忠[1] 李晓栩[1] 唐大运[1] 白波[1] 吴桂贤[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530005

出　　处：《动物医学进展》2009年第3期22-27,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30660140);广西教育厅科研项目:桂教科研(200620)

摘　　要：利用伊氏锥虫的优势变异型虫体作为可溶性抗原,通过试验研究和条件优化,建立了一种水牛伊氏锥虫间接ELISA方法。测试结果表明,该法灵敏度高,特异性强,对实验感染样本的检测获得了较好的效果,为一种有效的ELIAS方法,为广西水牛伊氏锥虫病的诊断奠定了技术基础。A dominant VAT（variant antigen type） of Trypanosorna evansi was usued as solube antigen to establish an indirect ELISA for Trypanosoma evansi in water buffaloes. The results indicated that the established method had very high sensitivity, specitivity,and good effects were obtained by using this method to detect experimentally infected samples. This research set up a base for further establishing an indirect ELISA for Trypanosoma evansi in clinical use.

关 键 词：伊氏锥虫 水牛 酶联免疫吸附试验 

分 类 号：S858.23[农业科学—临床兽医学] S852.72[农业科学—兽医学]