电堆积-非水毛细管电泳法同时分离测定虎杖中的有效成分  

Studies on separation and determination of three extracts in polygoni cuspidati with sample stacking-non-aqueous electrophoresis

作　　者：李利军[1] 吴峰敏[2,3] 李斯光[3] 喻来波[3] 吴健玲[3] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]河南科技大学化工与制药学院,洛阳471003 [3]广西大学化学与化学工程学院,南宁530005

出　　处：《分析试验室》2009年第4期55-58,共4页Chinese Journal of Analysis Laboratory

基　　金：广西自然科学基金(桂科自0146001);广西教育厅科学研究基金(桂教科[2000]392)项目资助

摘　　要：建立了电堆积富集-非水毛细管电泳同时分离测定中药虎杖中的大黄素、白藜芦醇和虎杖苷的新方法。对各种影响因素做了系统的研究,确立了中药虎杖中3种有效成分的最佳电堆积和分离条件,以甲醇为非水介质,30mmol/L NaAc-4 mmol/L NaOH-2 mmol/L CTAB溶液为背景电解质,运行电压为-25 kV,在220 nm波长下紫外检测。该法已应用于中药虎杖中大黄素、白藜芦醇和虎杖苷同时分离测定。A new method for the simultaneous determination of emodin, resveratrol and resveratrol-3-O-β-D-glucoside by stacking-non-aqueous-capillary electrophoresis had been proposed. The operating buffer was composed of 30 mmol/L sodium acetate-4 mmol/L sodium hydroxide-2 mmol/L CTMAB in methanol. The applied voltage was - 25 kV and the UV detection wavelength was 220 nm. This method is simple, rapid and accurate. It had been successfully used for the determination of three extracts in pelygoni cuspidati.

关 键 词：电堆积 非水毛细管电泳 大黄素 白藜芦醇 虎杖苷 虎杖 

分 类 号：O557.8[理学—热学与物质分子运动论]