莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞钙超载和细胞毒  

Morroniside Inhibits Hydrogen Peroxide-induced Calcium Overload and Cytotoxicity in SH-SY5Y Neuroblastoma Cells

作　　者：王文[1] 孙芳玲[1] 安宜[2] 黄文婷[2] 艾厚喜[1] 张丽[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室,北京市100053 [2]北京理工大学生命科学与技术学院生物技术系,北京市100081

出　　处：《中国康复理论与实践》2009年第3期201-202,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(30740053);北京市自然科学基金项目(7062031);国家“973”项目(2003CB517104);首都医科大学基础临床课题(2006JL09);教育部神经病性疾病学重点实验室课题(200704、200806)

摘　　要：目的研究莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞钙超载和细胞毒作用。方法体外培养的人神经母细胞瘤细胞系SH-SY5Y细胞分别加入莫诺苷1μmol/L、10μmol/L、100μmol/L预孵育24h,加入H2O2300～500μmol/L,作用18h,检测细胞内游离钙离子浓度、乳酸脱氢酶(LDH)释放率。结果与未加莫诺苷预孵育的细胞相比,莫诺苷10μmol/L、100μmol/L能抑制神经细胞内游离钙离子浓度增加,莫诺苷1μmol/L、10μmol/L、100μmol/L能降低LDH的释放率。结论莫诺苷能够抑制H2O2诱导的SH-SY5Y神经细胞钙超载和细胞毒,有神经保护作用。Objective To investigate the effects of morroniside on hydrogen peroxide （H2O2 ）-induced calcium overload and cyto- toxicity in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside 1, 10, or 100 μmol/L for 24 h prior to exposure to H2O2 300-500 μmol/L for 18 h. The cytosolic free calcium coacentration and LDH release were determined. Results The H2O2-induced cytosolic free calcium concentration decreased in the cells pre-incubated with morroniside 10 or 100 μmol/L, while the LDH release level decreased in the cells pre-incubated with morroniside 1, 10 or 100 μmol/L, comparison with the cells exposed H2O2 along. Conclusion Morroniside effects neuroprotection against H2O2-induced calcium overload and cyto- toxicity in SH-SY5Y cell.

关 键 词：莫诺苷 人神经母细胞瘤细胞 SH-SY5Y系 氧化应激 钙超载 细胞毒 

分 类 号：R742[医药卫生—神经病学与精神病学]