探索环境对局灶性脑梗死大鼠行为学恢复及梗死灶周围皮层神经颗粒素表达的影响  

Effect of Exploratory Environment on Behavioral Recovery and Expression of Neurogranin in Rats after Unilateral Local Cerebral Infarction

作　　者：槐雅萍 贾新凤[2] 贾子善[1] 

机构地区：[1]河北省人民医院康复医学科,河北石家庄市050051 [2]天津大港油田总医院,天津市300280

出　　处：《中国康复理论与实践》2009年第3期220-222,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：河北省科技攻关课题(07276101D-3)

摘　　要：目的观察探索环境对局灶性脑梗死大鼠行为学恢复及神经颗粒素(Ng)表达的影响。方法健康雄性SD大鼠70只,其中60只电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索组(n=30,置于迷宫笼喂养)和手术对照组(n=30,置于普通宠喂养),另10只为假手术组。各实验组随机取10只,分别于术后1d、7d、14d、28d时进行Berderson神经功能评分、肌力试验及平衡木试验。探索组和手术对照组分别于术后1d、7d、14d、28d处死5只大鼠,假手术组分别于7d、28d时处死5只大鼠,进行Ng免疫组化染色。结果探索组行为学评分于14～28d优于手术对照组(P<0.05)。Ng阳性神经元数在7d、14d、28d明显多于手术对照组(P<0.01)。结论探索环境可促进大鼠行为学功能的恢复并促进梗死灶周围皮层Ng表达。Objective To study the effect of exploratory environment on behavioral recovery and the expression of neurogranin （Ng） in rats after unilateral local cerebral infarction. Methods 70 male Sprague-Dawley rats were adopted. After making the model of MCAO with electric coagulation, 60 rats were randomly divided into exploratory group （n=30, living in maze cages） and control group （n=30, living in large standard cages）. Other 10 rats as the sham group lived in standard cage. 10 rats were chosen randomly from the groups for Berderson test, manual muscle and balancing test on the 1st, 7th, 14th, 28th day after operation, and 5 rats were sacrificed separately in exploratory group and control group at the same time. 5 rats in sham group were randomly sacrificed separately on the ？th, 28th day after operation. The expressions of Ng in the peri-ischemic cortex were detected with immunohisto- chemistry stain. Results The scores of behavior in exploratory group improved compared with that in control group （P〈0.05） from 14 d after operation. The number of Ng positive cells increased in exploratory group compared with that in control group （P^0.01） from 7 d after operation. Conclusion Exploratory environment can facilitate the functional recovery and the Ng expression in rats after unilateral local cerebral infarction.

关 键 词：探索环境 脑梗死 行为 神经颗粒素(Ng) 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]