5-烯丙基-7-二氟亚甲基白杨素抑制人卵巢癌裸鼠移植瘤生长及促凋亡作用  

5-allyl-7-gen-difluoromethylenechrysin inhibits the growth of human ovarian carcinoma transplanted subcutaneously and promotes its apoptosis in nude mice

作　　者：王淑芬[1] 谢宛玉[1] 郑兴[2] 曹建国[2] 

机构地区：[1]南华大学附属第一医院 [2]湖南师范大学医学院医药学实验管理中心,湖南长沙410000

出　　处：《中国现代医学杂志》2009年第5期680-683,共4页China Journal of Modern Medicine

基　　金：湖南省科技厅科研项目(No:06FJ3125)

摘　　要：目的观察5-烯丙基-7-二氟亚甲基白杨素(ADFMChR)对人卵巢癌CoC1裸鼠移植瘤生长的抑制及促凋亡作用。方法建立人卵巢癌CoC1裸鼠皮下移植瘤模型,随机分成5组:生理盐水组、顺铂组(2mg/kg)、低剂量ADFMChR组(6mg/kg)、中剂量ADFMChR组(18mg/kg)和高剂量ADFMChR组(54mg/kg),每组4只。观察各组裸鼠移植瘤生长情况、裸鼠体重的变化;PI染色流式细胞术(FCM)测定移植瘤细胞凋亡率。结果ADFMChR对移植瘤的生长有明显抑制作用,低剂量组、中剂量组和高剂量组的瘤重抑制率分别达62.76%、91.76%和77.55%。PI流式细胞术结果显示ADFMChR诱导移植瘤细胞凋亡,呈剂量依赖性。结论ADFMChR具有抑制人卵巢癌裸鼠移植瘤生长的作用并能诱导移植瘤细胞凋亡。[Objective] To investigate the inhibitory effects of 5-allyl-7-gen--difluoromethylene- chrysin （ADFMChR） on the growth of human ovarian carcinoma xenografts and its promotion on apoptosis in nude mice. [Methods] Model of human ovarian cancer transplanted subcutaneously in nude mice was established, and divided into 5 groups： control group, DDP （2 mg/kg） group, low dose ADFMChR （6 mg/kg） group, media dose ADFMChR （18 mg/kg） group and high dose ADFMChR （54 mg/kg） group. Tumor volume and weight were measured. The rate of apoptotie cells of human ovarian carcinoma xenografts was analyzed by PI stained flow eytometry （FCM）. [Results] ADFMChR could signifieandy suppress tumor growth of human ovarian carcinoma xenografts in mouse models. After treatment with ADFMChR （6, 18 and 54 mg/kg）, the inhibitory rates of the weight of human ovarian carcinoma xenografts were 42.86%, 62.76% and 77.55% respectively. [Conclusion] ADFMChR suppresses implanted tumor of human ovarian carcinoma ceils in nude mice through inducing apoptosis.

关 键 词：卵巢肿瘤 5-烯丙基-7-二氟亚甲基白杨素 移植瘤 凋亡 

分 类 号：R730.53[医药卫生—肿瘤]